l-Lysine production by exponential feeding of l-threonine

The effect of l-threonine feeding in the production phase on l-lysine production by Brevibacterium flavum, which requires l-homoserine or l-threonine for cell growth, was investigated considering the concerted inhibition by l-threonine plus l-lysine, and the metabolism related to lysine production....

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2000, Vol.90 (6), p.669-674
Main Authors: Tada, Kiyoshi, Kishimoto, Michimasa, Omasa, Takeshi, Katakura, Yoshio, Suga, Ken-Ichi
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
BREVIBACTERIUM
Brevibacterium flavum
exponential feeding
Fundamental and applied biological sciences. Psychology
l-lysine
l-threonine
LYSINE
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
THREONINE
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Summary:The effect of l-threonine feeding in the production phase on l-lysine production by Brevibacterium flavum, which requires l-homoserine or l-threonine for cell growth, was investigated considering the concerted inhibition by l-threonine plus l-lysine, and the metabolism related to lysine production. Exponential feeding of l-threonine increased l-lysine production to 70 g/ l about three times that without feeding. From the analysis of the metabolic flux, carbon flux of l-lysine synthesis pathway in the production phase after l-threonine feeding was higher than that in the growth phase. The results show that feeding of an inhibitory substance may increase the production, especially when the substance is necessary for the continuation of cell growth and/or production.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(00)90015-X