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The influence of polyunsaturated fatty acids on probiotic growth and adhesion
The establishment of the intestinal microflora, and probiotic bacteria, may control the inflammatory conditions in the gut. As polyunsaturated fatty acids (PUFA) possess antimicrobial activities, they may deter the action of probiotics. We assessed whether free linoleic, γ-linolenic, arachidonic, α-...
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| Published in: | FEMS microbiology letters 2001-01, Vol.194 (2), p.149-153 |
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| container_title | FEMS microbiology letters |
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| creator | Kankaanpää, Pasi E. Salminen, Seppo J. Isolauri, Erika Lee, Yuan Kun |
| description | The establishment of the intestinal microflora, and probiotic bacteria, may control the inflammatory conditions in the gut. As polyunsaturated fatty acids (PUFA) possess antimicrobial activities, they may deter the action of probiotics. We assessed whether free linoleic, γ-linolenic, arachidonic, α-linolenic and docosahexaenoic acids at physiological concentrations in the growth media would influence the growth and adhesion of
Lactobacillus GG (probiotic),
Lactobacillus casei Shirota (probiotic) and
Lactobacillus bulgaricus (dairy strain). Higher concentrations of PUFA (10–40 μg PUFA ml
−1) inhibited growth and mucus adhesion of all tested bacterial strains, whilst growth and mucus adhesion of
L. casei Shirota was promoted by low concentrations of γ-linolenic acid and arachidonic acid (at 5 μg ml
−1), respectively. PUFA also altered bacterial adhesion sites on Caco-2 cells. Caco-2 cells grown in the presence of arachidonic acid were less adhered to by all three bacterial strains. Yet,
L. casei Shirota adhered better on Caco-2 cells grown in the presence of α-linolenic acid. As the adhesion to mucosal surfaces is pivotal in health promoting effects by probiotics, our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA. |
| doi_str_mv | 10.1016/S0378-1097(00)00519-X |
| format | article |
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Lactobacillus GG (probiotic),
Lactobacillus casei Shirota (probiotic) and
Lactobacillus bulgaricus (dairy strain). Higher concentrations of PUFA (10–40 μg PUFA ml
−1) inhibited growth and mucus adhesion of all tested bacterial strains, whilst growth and mucus adhesion of
L. casei Shirota was promoted by low concentrations of γ-linolenic acid and arachidonic acid (at 5 μg ml
−1), respectively. PUFA also altered bacterial adhesion sites on Caco-2 cells. Caco-2 cells grown in the presence of arachidonic acid were less adhered to by all three bacterial strains. Yet,
L. casei Shirota adhered better on Caco-2 cells grown in the presence of α-linolenic acid. As the adhesion to mucosal surfaces is pivotal in health promoting effects by probiotics, our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(00)00519-X</identifier><identifier>PMID: 11164299</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>a-Linolenic acid ; Adhesion ; alpha-linolenic acid ; anti-infective properties ; arachidonic acid ; bacterial adhesion ; Bacterial Adhesion - drug effects ; Bacteriology ; Biological and medical sciences ; Caco-2 cell ; Caco-2 Cells ; Cell Adhesion - drug effects ; culture media ; digestive system ; docosahexaenoic acid ; Fatty Acids, Unsaturated - pharmacology ; Fundamental and applied biological sciences. Psychology ; g-Linolenic acid ; gamma-linolenic acid ; growth retardation ; health promotion ; human cell lines ; Humans ; intestinal microorganisms ; Intestinal mucus ; Lactobacillus ; Lactobacillus - drug effects ; Lactobacillus - growth & development ; Lactobacillus bulgaricus ; Lactobacillus casei ; Lactobacillus delbrueckii subsp. bulgaricus ; linoleic acid ; Microbiology ; mucosa ; mucus ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Polyunsaturated fatty acid ; Probiotic bacterium ; probiotics ; Probiotics - metabolism</subject><ispartof>FEMS microbiology letters, 2001-01, Vol.194 (2), p.149-153</ispartof><rights>2001 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-34fb634f2d4bffe15327d3d029fcaa4f668e1c9d39ff3914008c8e05b1a96b543</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14226023$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11164299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kankaanpää, Pasi E.</creatorcontrib><creatorcontrib>Salminen, Seppo J.</creatorcontrib><creatorcontrib>Isolauri, Erika</creatorcontrib><creatorcontrib>Lee, Yuan Kun</creatorcontrib><title>The influence of polyunsaturated fatty acids on probiotic growth and adhesion</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>The establishment of the intestinal microflora, and probiotic bacteria, may control the inflammatory conditions in the gut. As polyunsaturated fatty acids (PUFA) possess antimicrobial activities, they may deter the action of probiotics. We assessed whether free linoleic, γ-linolenic, arachidonic, α-linolenic and docosahexaenoic acids at physiological concentrations in the growth media would influence the growth and adhesion of
Lactobacillus GG (probiotic),
Lactobacillus casei Shirota (probiotic) and
Lactobacillus bulgaricus (dairy strain). Higher concentrations of PUFA (10–40 μg PUFA ml
−1) inhibited growth and mucus adhesion of all tested bacterial strains, whilst growth and mucus adhesion of
L. casei Shirota was promoted by low concentrations of γ-linolenic acid and arachidonic acid (at 5 μg ml
−1), respectively. PUFA also altered bacterial adhesion sites on Caco-2 cells. Caco-2 cells grown in the presence of arachidonic acid were less adhered to by all three bacterial strains. Yet,
L. casei Shirota adhered better on Caco-2 cells grown in the presence of α-linolenic acid. As the adhesion to mucosal surfaces is pivotal in health promoting effects by probiotics, our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA.</description><subject>a-Linolenic acid</subject><subject>Adhesion</subject><subject>alpha-linolenic acid</subject><subject>anti-infective properties</subject><subject>arachidonic acid</subject><subject>bacterial adhesion</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Caco-2 cell</subject><subject>Caco-2 Cells</subject><subject>Cell Adhesion - drug effects</subject><subject>culture media</subject><subject>digestive system</subject><subject>docosahexaenoic acid</subject><subject>Fatty Acids, Unsaturated - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>g-Linolenic acid</subject><subject>gamma-linolenic acid</subject><subject>growth retardation</subject><subject>health promotion</subject><subject>human cell lines</subject><subject>Humans</subject><subject>intestinal microorganisms</subject><subject>Intestinal mucus</subject><subject>Lactobacillus</subject><subject>Lactobacillus - drug effects</subject><subject>Lactobacillus - growth & development</subject><subject>Lactobacillus bulgaricus</subject><subject>Lactobacillus casei</subject><subject>Lactobacillus delbrueckii subsp. bulgaricus</subject><subject>linoleic acid</subject><subject>Microbiology</subject><subject>mucosa</subject><subject>mucus</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Polyunsaturated fatty acid</subject><subject>Probiotic bacterium</subject><subject>probiotics</subject><subject>Probiotics - metabolism</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqF0TtPHDEQB3ALEYULyUcguCFKiiXjx3rXFYpQXhIRBSDRWV4_OEd768P2JrpvHx93CiUu7OY3ntF_EDohcE6AiM83wLq-ISC7jwCfAFoim_sDtCBtxxshRX-IFv_JEXqT828A4BTEa3RECBGcSrlAv26XDofJj7ObjMPR43UcN_OUdZmTLs5ir0vZYG2CzThOeJ3iEGIJBj-k-LcssZ4s1nbpcojTW_TK6zG7d_v3GN19-3p7-aO5uv7-8_LLVWNaQkvDuB9Evajlg_eOtIx2llmg0hutuReid8RIy6T3TBIO0JveQTsQLcXQcnaMPuz-rdM8zi4XtQrZuHHUk4tzVh0IILKHFyHp6mFMVNjuoEkx5-S8Wqew0mmjCKht4OopcLVNUwGop8DVfa17v28wDytnn6v2CVdwtgc6Gz36pCcT8rPjlAqgrLrTnfM6Kv2Qqrm7obVx3ZoUgvdVXOyEq8n-CS6pbMJ2azYkZ4qyMbww7D-L3aZd</recordid><startdate>20010115</startdate><enddate>20010115</enddate><creator>Kankaanpää, Pasi E.</creator><creator>Salminen, Seppo J.</creator><creator>Isolauri, Erika</creator><creator>Lee, Yuan Kun</creator><general>Elsevier B.V</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20010115</creationdate><title>The influence of polyunsaturated fatty acids on probiotic growth and adhesion</title><author>Kankaanpää, Pasi E. ; Salminen, Seppo J. ; Isolauri, Erika ; Lee, Yuan Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-34fb634f2d4bffe15327d3d029fcaa4f668e1c9d39ff3914008c8e05b1a96b543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>a-Linolenic acid</topic><topic>Adhesion</topic><topic>alpha-linolenic acid</topic><topic>anti-infective properties</topic><topic>arachidonic acid</topic><topic>bacterial adhesion</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Caco-2 cell</topic><topic>Caco-2 Cells</topic><topic>Cell Adhesion - drug effects</topic><topic>culture media</topic><topic>digestive system</topic><topic>docosahexaenoic acid</topic><topic>Fatty Acids, Unsaturated - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>g-Linolenic acid</topic><topic>gamma-linolenic acid</topic><topic>growth retardation</topic><topic>health promotion</topic><topic>human cell lines</topic><topic>Humans</topic><topic>intestinal microorganisms</topic><topic>Intestinal mucus</topic><topic>Lactobacillus</topic><topic>Lactobacillus - drug effects</topic><topic>Lactobacillus - growth & development</topic><topic>Lactobacillus bulgaricus</topic><topic>Lactobacillus casei</topic><topic>Lactobacillus delbrueckii subsp. bulgaricus</topic><topic>linoleic acid</topic><topic>Microbiology</topic><topic>mucosa</topic><topic>mucus</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Polyunsaturated fatty acid</topic><topic>Probiotic bacterium</topic><topic>probiotics</topic><topic>Probiotics - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kankaanpää, Pasi E.</creatorcontrib><creatorcontrib>Salminen, Seppo J.</creatorcontrib><creatorcontrib>Isolauri, Erika</creatorcontrib><creatorcontrib>Lee, Yuan Kun</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kankaanpää, Pasi E.</au><au>Salminen, Seppo J.</au><au>Isolauri, Erika</au><au>Lee, Yuan Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The influence of polyunsaturated fatty acids on probiotic growth and adhesion</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2001-01-15</date><risdate>2001</risdate><volume>194</volume><issue>2</issue><spage>149</spage><epage>153</epage><pages>149-153</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>The establishment of the intestinal microflora, and probiotic bacteria, may control the inflammatory conditions in the gut. As polyunsaturated fatty acids (PUFA) possess antimicrobial activities, they may deter the action of probiotics. We assessed whether free linoleic, γ-linolenic, arachidonic, α-linolenic and docosahexaenoic acids at physiological concentrations in the growth media would influence the growth and adhesion of
Lactobacillus GG (probiotic),
Lactobacillus casei Shirota (probiotic) and
Lactobacillus bulgaricus (dairy strain). Higher concentrations of PUFA (10–40 μg PUFA ml
−1) inhibited growth and mucus adhesion of all tested bacterial strains, whilst growth and mucus adhesion of
L. casei Shirota was promoted by low concentrations of γ-linolenic acid and arachidonic acid (at 5 μg ml
−1), respectively. PUFA also altered bacterial adhesion sites on Caco-2 cells. Caco-2 cells grown in the presence of arachidonic acid were less adhered to by all three bacterial strains. Yet,
L. casei Shirota adhered better on Caco-2 cells grown in the presence of α-linolenic acid. As the adhesion to mucosal surfaces is pivotal in health promoting effects by probiotics, our results indicate that the action of probiotics in the gut may be modulated by dietary PUFA.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>11164299</pmid><doi>10.1016/S0378-1097(00)00519-X</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2001-01, Vol.194 (2), p.149-153 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | a-Linolenic acid Adhesion alpha-linolenic acid anti-infective properties arachidonic acid bacterial adhesion Bacterial Adhesion - drug effects Bacteriology Biological and medical sciences Caco-2 cell Caco-2 Cells Cell Adhesion - drug effects culture media digestive system docosahexaenoic acid Fatty Acids, Unsaturated - pharmacology Fundamental and applied biological sciences. Psychology g-Linolenic acid gamma-linolenic acid growth retardation health promotion human cell lines Humans intestinal microorganisms Intestinal mucus Lactobacillus Lactobacillus - drug effects Lactobacillus - growth & development Lactobacillus bulgaricus Lactobacillus casei Lactobacillus delbrueckii subsp. bulgaricus linoleic acid Microbiology mucosa mucus Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polyunsaturated fatty acid Probiotic bacterium probiotics Probiotics - metabolism |
| title | The influence of polyunsaturated fatty acids on probiotic growth and adhesion |
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