Accumulation of a recombinant Aspergillus oryzae lipase artificially localized on the Bacillus subtilis cell surface

cutL cDNA encoding an extracellular lipase, L1, from Aspergillus oryzae was fused to the cell wall-binding domain (CWB) region of a plasmid, pHCB3R. SDS-polyacrylamide gel electrophoresis (PAGE) and zymography of proteins extracted from the cell surface of Bacillus subtilis 168 harboring a fused lip...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2000, Vol.90 (4), p.422-425
Main Authors: Kobayashi, Gota, Toida, Jinichi, Akamatsu, Takashi, Yamamoto, Hiroki, Shida, Toshio, Sekiguchi, Junichi
Format: Article
Language:English
Subjects:
ASPERGILLUS ORYZAE
autolysin
BACILLUS SUBTILIS
Biological and medical sciences
Biotechnology
CELL WALLS
Cells
DNA
Electrophoresis
ESTERASES
Fundamental and applied biological sciences. Psychology
Genes
Genetic engineering
Genetic technics
lipase
Methods. Procedures. Technologies
Modification of gene expression level
Proteins
surface engineering
Surfaces
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Summary:cutL cDNA encoding an extracellular lipase, L1, from Aspergillus oryzae was fused to the cell wall-binding domain (CWB) region of a plasmid, pHCB3R. SDS-polyacrylamide gel electrophoresis (PAGE) and zymography of proteins extracted from the cell surface of Bacillus subtilis 168 harboring a fused lipase plasmid (pHCB3RCL) revealed that the fused gene product, CWB-CutL, was localized in the B. subtilis cell wall and retained lipase activity. B. subtilis WASD ( wprA sigD), recently used for the accumulation of CWB-LipB (the CWB protein fused with B. subtilis lipase B), was also a suitable host for the accumulation of CWB-CutL, the amount being 10% of the total proteins extracted from the cell surface.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(01)80012-8