Loading…
Upregulation of Wilms’ tumour gene 1 ( WT1 ) in uterine sarcomas
Abstract Aim Overexpression of Wilms’ tumour gene ( WT1 ) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1 . We therefore intended to further explore the expression pattern o...
Saved in:
| Published in: | European journal of cancer (1990) 2007-07, Vol.43 (10), p.1630-1637 |
|---|---|
| Main Authors: | , , , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c514t-9749b99296061db4bb865a058e8c6d326ff9e69722bfdc48eabb6e08134182663 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c514t-9749b99296061db4bb865a058e8c6d326ff9e69722bfdc48eabb6e08134182663 |
| container_end_page | 1637 |
| container_issue | 10 |
| container_start_page | 1630 |
| container_title | European journal of cancer (1990) |
| container_volume | 43 |
| creator | Coosemans, A Nik, S. Amini Caluwaerts, S Lambin, S Verbist, G Van Bree, R Schelfhout, V de Jonge, E Dalle, I Jacomen, G Cassiman, J.J Moerman, Ph Vergote, I Amant, F |
| description | Abstract Aim Overexpression of Wilms’ tumour gene ( WT1 ) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1 . We therefore intended to further explore the expression pattern of WT1 in uterine sarcomas. Methods 27 CS, 38 LMS, 15 endometrial stromal sarcomas (ESS) and seven undifferentiated sarcomas (US) were collected. WT1 expression was evaluated by immunohistochemistry (IHC) in 87 samples, by RT-PCR (m-RNA expression) in 23 random selected samples and by Western blotting in 12 samples, separating cytoplasmic and nuclear proteins. A pilot study to detect mutations (exons 7–10) was performed on eight samples. Results IHC showed WT1 positivity in 12/27 CS, 29/38 LMS, 7/15 ESS and 4/7 US. All-but-one sample had a positive RT-PCR. All Western blottings were positive with more cytoplasmic expression in 9/12 cases. No mutations were found. Conclusions WT1 is overexpressed in uterine sarcomas. Since increased levels of mRNA determine the biological role, WT1 might contribute to uterine sarcoma tumour biology. |
| doi_str_mv | 10.1016/j.ejca.2007.04.008 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70606244</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0959804907002948</els_id><sourcerecordid>70606244</sourcerecordid><originalsourceid>FETCH-LOGICAL-c514t-9749b99296061db4bb865a058e8c6d326ff9e69722bfdc48eabb6e08134182663</originalsourceid><addsrcrecordid>eNqFks9qFTEUh4NY7PXqC7iQbBRdzHiSyWQSEKEt2goFF7Z0GTKZMyXj_LlNZoTufA1fzydphnuh4EJXgcN3fid85xDyikHOgMkPXY6dszkHqHIQOYB6QjZMVToDVfKnZAO61JkCoY_J8xg7SKAS8Iwcs6osmJDVhpxe7wLeLr2d_TTSqaU3vh_in1-_6bwM0xLoLY5IGX1Hb64YfU_9SJcZg0_FaIObBhtfkKPW9hFfHt4tuf7y-ersIrv8dv717OQycyUTc6YroWutuZYgWVOLulaytFAqVE42BZdtq1HqivO6bZxQaOtaIihWCKa4lMWWvN3n7sJ0t2CczeCjw763I05LNBWkZC7Ef0GmkwVdrYl8D7owxRiwNbvgBxvuDQOzKjadWRWbVbEBYZLi1PT6kL7UAzaPLQenCXhzAGx0tm-DHZ2Pj5xSaXSCt-TjnsMk7afHYKLzODpsfEA3m2by__7Hp7_aXe9Hnyb-wHuMXVremNZhmIncgPm-HsN6C1ABcC1U8QCl9qyJ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19840976</pqid></control><display><type>article</type><title>Upregulation of Wilms’ tumour gene 1 ( WT1 ) in uterine sarcomas</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Coosemans, A ; Nik, S. Amini ; Caluwaerts, S ; Lambin, S ; Verbist, G ; Van Bree, R ; Schelfhout, V ; de Jonge, E ; Dalle, I ; Jacomen, G ; Cassiman, J.J ; Moerman, Ph ; Vergote, I ; Amant, F</creator><creatorcontrib>Coosemans, A ; Nik, S. Amini ; Caluwaerts, S ; Lambin, S ; Verbist, G ; Van Bree, R ; Schelfhout, V ; de Jonge, E ; Dalle, I ; Jacomen, G ; Cassiman, J.J ; Moerman, Ph ; Vergote, I ; Amant, F</creatorcontrib><description>Abstract Aim Overexpression of Wilms’ tumour gene ( WT1 ) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1 . We therefore intended to further explore the expression pattern of WT1 in uterine sarcomas. Methods 27 CS, 38 LMS, 15 endometrial stromal sarcomas (ESS) and seven undifferentiated sarcomas (US) were collected. WT1 expression was evaluated by immunohistochemistry (IHC) in 87 samples, by RT-PCR (m-RNA expression) in 23 random selected samples and by Western blotting in 12 samples, separating cytoplasmic and nuclear proteins. A pilot study to detect mutations (exons 7–10) was performed on eight samples. Results IHC showed WT1 positivity in 12/27 CS, 29/38 LMS, 7/15 ESS and 4/7 US. All-but-one sample had a positive RT-PCR. All Western blottings were positive with more cytoplasmic expression in 9/12 cases. No mutations were found. Conclusions WT1 is overexpressed in uterine sarcomas. Since increased levels of mRNA determine the biological role, WT1 might contribute to uterine sarcoma tumour biology.</description><identifier>ISSN: 0959-8049</identifier><identifier>EISSN: 1879-0852</identifier><identifier>DOI: 10.1016/j.ejca.2007.04.008</identifier><identifier>PMID: 17531467</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Blotting, Western ; Cytoplasmic expression ; DNA Mutational Analysis ; DNA, Neoplasm - analysis ; Female ; Genes, Wilms Tumor ; Hematology, Oncology and Palliative Medicine ; Humans ; Immunohistochemistry ; Medical sciences ; Mutation - genetics ; Pharmacology. Drug treatments ; Reverse Transcriptase Polymerase Chain Reaction ; Sarcoma ; Sarcoma - genetics ; Tumors ; Up-Regulation ; Uterine Neoplasms - genetics ; Uterus ; Wilms’ tumour gene 1 ; WT1</subject><ispartof>European journal of cancer (1990), 2007-07, Vol.43 (10), p.1630-1637</ispartof><rights>Elsevier Ltd</rights><rights>2007 Elsevier Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-9749b99296061db4bb865a058e8c6d326ff9e69722bfdc48eabb6e08134182663</citedby><cites>FETCH-LOGICAL-c514t-9749b99296061db4bb865a058e8c6d326ff9e69722bfdc48eabb6e08134182663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18876375$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17531467$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coosemans, A</creatorcontrib><creatorcontrib>Nik, S. Amini</creatorcontrib><creatorcontrib>Caluwaerts, S</creatorcontrib><creatorcontrib>Lambin, S</creatorcontrib><creatorcontrib>Verbist, G</creatorcontrib><creatorcontrib>Van Bree, R</creatorcontrib><creatorcontrib>Schelfhout, V</creatorcontrib><creatorcontrib>de Jonge, E</creatorcontrib><creatorcontrib>Dalle, I</creatorcontrib><creatorcontrib>Jacomen, G</creatorcontrib><creatorcontrib>Cassiman, J.J</creatorcontrib><creatorcontrib>Moerman, Ph</creatorcontrib><creatorcontrib>Vergote, I</creatorcontrib><creatorcontrib>Amant, F</creatorcontrib><title>Upregulation of Wilms’ tumour gene 1 ( WT1 ) in uterine sarcomas</title><title>European journal of cancer (1990)</title><addtitle>Eur J Cancer</addtitle><description>Abstract Aim Overexpression of Wilms’ tumour gene ( WT1 ) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1 . We therefore intended to further explore the expression pattern of WT1 in uterine sarcomas. Methods 27 CS, 38 LMS, 15 endometrial stromal sarcomas (ESS) and seven undifferentiated sarcomas (US) were collected. WT1 expression was evaluated by immunohistochemistry (IHC) in 87 samples, by RT-PCR (m-RNA expression) in 23 random selected samples and by Western blotting in 12 samples, separating cytoplasmic and nuclear proteins. A pilot study to detect mutations (exons 7–10) was performed on eight samples. Results IHC showed WT1 positivity in 12/27 CS, 29/38 LMS, 7/15 ESS and 4/7 US. All-but-one sample had a positive RT-PCR. All Western blottings were positive with more cytoplasmic expression in 9/12 cases. No mutations were found. Conclusions WT1 is overexpressed in uterine sarcomas. Since increased levels of mRNA determine the biological role, WT1 might contribute to uterine sarcoma tumour biology.</description><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cytoplasmic expression</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Neoplasm - analysis</subject><subject>Female</subject><subject>Genes, Wilms Tumor</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Mutation - genetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sarcoma</subject><subject>Sarcoma - genetics</subject><subject>Tumors</subject><subject>Up-Regulation</subject><subject>Uterine Neoplasms - genetics</subject><subject>Uterus</subject><subject>Wilms’ tumour gene 1</subject><subject>WT1</subject><issn>0959-8049</issn><issn>1879-0852</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFks9qFTEUh4NY7PXqC7iQbBRdzHiSyWQSEKEt2goFF7Z0GTKZMyXj_LlNZoTufA1fzydphnuh4EJXgcN3fid85xDyikHOgMkPXY6dszkHqHIQOYB6QjZMVToDVfKnZAO61JkCoY_J8xg7SKAS8Iwcs6osmJDVhpxe7wLeLr2d_TTSqaU3vh_in1-_6bwM0xLoLY5IGX1Hb64YfU_9SJcZg0_FaIObBhtfkKPW9hFfHt4tuf7y-ersIrv8dv717OQycyUTc6YroWutuZYgWVOLulaytFAqVE42BZdtq1HqivO6bZxQaOtaIihWCKa4lMWWvN3n7sJ0t2CczeCjw763I05LNBWkZC7Ef0GmkwVdrYl8D7owxRiwNbvgBxvuDQOzKjadWRWbVbEBYZLi1PT6kL7UAzaPLQenCXhzAGx0tm-DHZ2Pj5xSaXSCt-TjnsMk7afHYKLzODpsfEA3m2by__7Hp7_aXe9Hnyb-wHuMXVremNZhmIncgPm-HsN6C1ABcC1U8QCl9qyJ</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Coosemans, A</creator><creator>Nik, S. Amini</creator><creator>Caluwaerts, S</creator><creator>Lambin, S</creator><creator>Verbist, G</creator><creator>Van Bree, R</creator><creator>Schelfhout, V</creator><creator>de Jonge, E</creator><creator>Dalle, I</creator><creator>Jacomen, G</creator><creator>Cassiman, J.J</creator><creator>Moerman, Ph</creator><creator>Vergote, I</creator><creator>Amant, F</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070701</creationdate><title>Upregulation of Wilms’ tumour gene 1 ( WT1 ) in uterine sarcomas</title><author>Coosemans, A ; Nik, S. Amini ; Caluwaerts, S ; Lambin, S ; Verbist, G ; Van Bree, R ; Schelfhout, V ; de Jonge, E ; Dalle, I ; Jacomen, G ; Cassiman, J.J ; Moerman, Ph ; Vergote, I ; Amant, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-9749b99296061db4bb865a058e8c6d326ff9e69722bfdc48eabb6e08134182663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cytoplasmic expression</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Neoplasm - analysis</topic><topic>Female</topic><topic>Genes, Wilms Tumor</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Mutation - genetics</topic><topic>Pharmacology. Drug treatments</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Sarcoma</topic><topic>Sarcoma - genetics</topic><topic>Tumors</topic><topic>Up-Regulation</topic><topic>Uterine Neoplasms - genetics</topic><topic>Uterus</topic><topic>Wilms’ tumour gene 1</topic><topic>WT1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coosemans, A</creatorcontrib><creatorcontrib>Nik, S. Amini</creatorcontrib><creatorcontrib>Caluwaerts, S</creatorcontrib><creatorcontrib>Lambin, S</creatorcontrib><creatorcontrib>Verbist, G</creatorcontrib><creatorcontrib>Van Bree, R</creatorcontrib><creatorcontrib>Schelfhout, V</creatorcontrib><creatorcontrib>de Jonge, E</creatorcontrib><creatorcontrib>Dalle, I</creatorcontrib><creatorcontrib>Jacomen, G</creatorcontrib><creatorcontrib>Cassiman, J.J</creatorcontrib><creatorcontrib>Moerman, Ph</creatorcontrib><creatorcontrib>Vergote, I</creatorcontrib><creatorcontrib>Amant, F</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cancer (1990)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coosemans, A</au><au>Nik, S. Amini</au><au>Caluwaerts, S</au><au>Lambin, S</au><au>Verbist, G</au><au>Van Bree, R</au><au>Schelfhout, V</au><au>de Jonge, E</au><au>Dalle, I</au><au>Jacomen, G</au><au>Cassiman, J.J</au><au>Moerman, Ph</au><au>Vergote, I</au><au>Amant, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upregulation of Wilms’ tumour gene 1 ( WT1 ) in uterine sarcomas</atitle><jtitle>European journal of cancer (1990)</jtitle><addtitle>Eur J Cancer</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>43</volume><issue>10</issue><spage>1630</spage><epage>1637</epage><pages>1630-1637</pages><issn>0959-8049</issn><eissn>1879-0852</eissn><abstract>Abstract Aim Overexpression of Wilms’ tumour gene ( WT1 ) has been proven in several tumours. Previous research of our group on the cell cycle of uterine leiomyosarcoma (LMS) and carcinosarcoma (CS) suggested a possible role for WT1 . We therefore intended to further explore the expression pattern of WT1 in uterine sarcomas. Methods 27 CS, 38 LMS, 15 endometrial stromal sarcomas (ESS) and seven undifferentiated sarcomas (US) were collected. WT1 expression was evaluated by immunohistochemistry (IHC) in 87 samples, by RT-PCR (m-RNA expression) in 23 random selected samples and by Western blotting in 12 samples, separating cytoplasmic and nuclear proteins. A pilot study to detect mutations (exons 7–10) was performed on eight samples. Results IHC showed WT1 positivity in 12/27 CS, 29/38 LMS, 7/15 ESS and 4/7 US. All-but-one sample had a positive RT-PCR. All Western blottings were positive with more cytoplasmic expression in 9/12 cases. No mutations were found. Conclusions WT1 is overexpressed in uterine sarcomas. Since increased levels of mRNA determine the biological role, WT1 might contribute to uterine sarcoma tumour biology.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>17531467</pmid><doi>10.1016/j.ejca.2007.04.008</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-8049 |
| ispartof | European journal of cancer (1990), 2007-07, Vol.43 (10), p.1630-1637 |
| issn | 0959-8049 1879-0852 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70606244 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Biological and medical sciences Blotting, Western Cytoplasmic expression DNA Mutational Analysis DNA, Neoplasm - analysis Female Genes, Wilms Tumor Hematology, Oncology and Palliative Medicine Humans Immunohistochemistry Medical sciences Mutation - genetics Pharmacology. Drug treatments Reverse Transcriptase Polymerase Chain Reaction Sarcoma Sarcoma - genetics Tumors Up-Regulation Uterine Neoplasms - genetics Uterus Wilms’ tumour gene 1 WT1 |
| title | Upregulation of Wilms’ tumour gene 1 ( WT1 ) in uterine sarcomas |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T18%3A22%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Upregulation%20of%20Wilms%E2%80%99%20tumour%20gene%201%20(%20WT1%20)%20in%20uterine%20sarcomas&rft.jtitle=European%20journal%20of%20cancer%20(1990)&rft.au=Coosemans,%20A&rft.date=2007-07-01&rft.volume=43&rft.issue=10&rft.spage=1630&rft.epage=1637&rft.pages=1630-1637&rft.issn=0959-8049&rft.eissn=1879-0852&rft_id=info:doi/10.1016/j.ejca.2007.04.008&rft_dat=%3Cproquest_cross%3E70606244%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c514t-9749b99296061db4bb865a058e8c6d326ff9e69722bfdc48eabb6e08134182663%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=19840976&rft_id=info:pmid/17531467&rfr_iscdi=true |