Establishment of a human herpes virus-8-negative malignant effusion lymphoma cell line (STR-428) carrying concurrent translocations of BCL2 and c-MYC genes

Abstract A new cell line, STR-428 was established from ascites tumor cells of a malignant effusion lymphoma patient without human herpes virus-8 (HHV-8) infection. STR-428 cells showed an immunophenotype of mature B-cells and produced few cytokines related to lymphomatous effusion. Karyotypic and ge...

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Bibliographic Details
Published in:Leukemia research 2007-09, Vol.31 (9), p.1285-1292
Main Authors: Taira, Tamiko, Nagasaki, Akitoshi, Tomoyose, Takeaki, Miyagi, Jun-ichi, Kakazu, Naoki, Makino, Shigeyoshi, Shinjyo, Tetsuharu, Taira, Naoya, Masuda, Masato, Takasu, Nobuyuki
Format: Article
Language:English
Subjects:
B-cell lymphoma
BCL2
Blotting, Southern
c-MYC
Cell line
Chromosomes, Human, Pair 14 - genetics
Chromosomes, Human, Pair 18 - genetics
Cytokines
Hematology, Oncology and Palliative Medicine
Herpesvirus 8, Human - physiology
Human herpes virus-8
Human herpesvirus 8
Humans
Immunoenzyme Techniques
Immunophenotyping
In Situ Hybridization, Fluorescence
Lymphoma - genetics
Lymphoma - metabolism
Lymphoma - virology
Male
Malignant effusion lymphoma
Middle Aged
Pleural Effusion, Malignant - genetics
Polymerase Chain Reaction
Primary effusion lymphoma
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-myc - genetics
Translocation, Genetic
Tumor Cells, Cultured
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Summary:Abstract A new cell line, STR-428 was established from ascites tumor cells of a malignant effusion lymphoma patient without human herpes virus-8 (HHV-8) infection. STR-428 cells showed an immunophenotype of mature B-cells and produced few cytokines related to lymphomatous effusion. Karyotypic and genetic analysis revealed complex translocations including t (14;18)(q32;q21) effecting IgH / BCL2 and der(8) t (3;8)(q27;q24) involving c-MYC . STR-428 represents a unique, B-cell lymphoma cell line carrying concurrent rearrangement of BCL2 and c-MYC genes with features distinct from those of HHV-8-related primary effusion lymphoma. This cell line may be a valuable tool, other than HHV-8, to investigate the pathogenesis of primary lymphomatous effusion.
ISSN:0145-2126
1873-5835
DOI:10.1016/j.leukres.2006.12.023