Precision of micellar electrokinetic capillary chromatography in the determination of seven antibiotics in pharmaceuticals and feedstuffs

Validation of analytical procedures is important for their efficient and reliable application. The International Conference on Harmonisation (ICH), Food and Drug Administration (FDA) and pharmacopoeia guidelines achieved a great deal in harmonising the definitions of the required validation characte...

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Bibliographic Details
Published in:Analytica chimica acta 2007-06, Vol.594 (1), p.119-127
Main Authors: Injac, Rade, Kocevar, Nina, Kreft, Samo
Format: Article
Language:English
Subjects:
Analytical chemistry
Animal Feed - analysis
Anti-Bacterial Agents - analysis
Antibiotics
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Micellar Electrokinetic Capillary - methods
Exact sciences and technology
Feedstuffes
General, instrumentation
Micellar electrokinetic capillary chromatography
Other chromatographic methods
Pharmaceutical Preparations - chemistry
Pharmaceuticals
Precision
Reproducibility of Results
Software
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Summary:Validation of analytical procedures is important for their efficient and reliable application. The International Conference on Harmonisation (ICH), Food and Drug Administration (FDA) and pharmacopoeia guidelines achieved a great deal in harmonising the definitions of the required validation characteristics. It is well known that poor reproducibility limits the practical implementation of capillary electrophoresis (CE). A precision study on four different MEKC methods was performed with 11 samples, containing seven antibiotics, by two analysts, in few days, on two capillary electrophoresis instruments. Five pharmaceutical preparations and three animal feeds were used. Precision was statistically analysed using migration time, peak area and height of each compound, as well as electroosmotic front (EOF). In 25 of 31 cases, the reproducibility of peak area, peak height and migration time was good (
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2007.05.003