An Inductively Coupled Plasma Carbon Emission Detector for Aqueous Carbohydrate Separations by Liquid Chromatography

An inductively coupled plasma atomic emission spectrometer is used to detect carbon-containing compounds following separation by high-performance liquid chromatography. A calcium form ligand exchange column with distilled and deionized water as the mobile phase is used to separate carbohydrates. The...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-02, Vol.73 (3), p.453-457
Main Authors: Peters, Heather L, Levine, Keith E, Jones, Bradley T
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Carbohydrates - isolation & purification
Carbon
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - methods
Emissions
Exact sciences and technology
Food industries
Fundamental and applied biological sciences. Psychology
Non alcoholic beverage industries and mineral waters
Other chromatographic methods
Sensitivity and Specificity
Spectrophotometry, Atomic - instrumentation
Spectrum analysis
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Summary:An inductively coupled plasma atomic emission spectrometer is used to detect carbon-containing compounds following separation by high-performance liquid chromatography. A calcium form ligand exchange column with distilled and deionized water as the mobile phase is used to separate carbohydrates. The eluting species are detected by monitoring the carbon atomic emission line at 193.09 nm. The mass detection limits using a photomultiplier tube for sucrose and glucose are 50 ng, while that for fructose is 60 ng. The carbon emission detector should provide the same detection limit for any compound with a similar mass percent of carbon, whether or not the compound exhibits appreciable absorption characteristics. While the carbon emission detector will universally detect any organic compound, it will discriminate against species with high molar absorptivity that may be present at low concentration. Such species may act as interferences in chromatograms generated with conventional UV−visible absorption detectors. To demonstrate the utility of the carbon emission detector, three sugars (glucose, fructose, sucrose) are determined in apple, crangrape, and orange juice.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac000902i