Protein adsorption and zeta potentials of a biphasic calcium phosphate ceramic under various conditions

An investigation on the relationship between protein adsorption and zeta potentials of a biphasic calcium phosphate (BCP) ceramic was carried out. Zeta potentials of the BCP ceramic particles were measured at various aqueous solutions. Bovine serum album (BSA) and its competitive adsorption with lys...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2007-07, Vol.82B (1), p.65-73
Main Authors: Zhu, Xiangdong, Fan, Hongsong, Li, Dongxiao, Xiao, Yumei, Zhang, Xingdong
Format: Article
Language:English
Subjects:
Adsorption
Animals
BCP ceramic
BSA
Calcium Phosphates - chemistry
Cattle
Ceramics - chemistry
Electrophoresis, Polyacrylamide Gel
electrostatic interaction
Hydrogen-Ion Concentration
LSZ
Microscopy, Electron, Scanning
Muramidase - analysis
Muramidase - chemistry
Osmolar Concentration
protein adsorption
Proteins - analysis
Proteins - chemistry
Serum Albumin, Bovine - analysis
Serum Albumin, Bovine - chemistry
Static Electricity
Surface Properties
zeta potential
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An investigation on the relationship between protein adsorption and zeta potentials of a biphasic calcium phosphate (BCP) ceramic was carried out. Zeta potentials of the BCP ceramic particles were measured at various aqueous solutions. Bovine serum album (BSA) and its competitive adsorption with lysozyme (LSZ) on BCP were investigated using conventional protein adsorption and polyacrylamide gel electrophoresis (PAGE) methods. The results showed that zeta potential and the amount of adsorbed BSA were both influenced by pH, ionic strength, Ca2+ and PO 43− concentrations in the buffers. The variation tendencies of BSA adsorption were consistent with that of zeta potentials to some extent. The co‐adsorption of BSA and LSZ on BCP was confirmed by the PAGE gel pattern. The semi‐quantitative analysis for the detected protein bands proved that LSZ had higher affinity for BCP than BSA and would preferentially bind to the surface. Electrostatic interaction played an important role in protein adsorption on the surface of the BCP ceramic particles. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.30706