Microbial population dynamics in a thermophilic methane digester fed with garbage

The diversity of microbial communities in three full-scale thermophilic anaerobic digesters which treated garbage, sewage sludge and livestock wastes (hereafter called TGD, TSD and TLD, respectively) was investigated using 16S rDNA clone libraries in triplicate. The population dynamics of TGD were a...

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Bibliographic Details
Published in:Water science and technology 2007-01, Vol.55 (10), p.175-182
Main Authors: Cheon, J, Hong, F, Hidaka, T, Koshikawa, H, Tsuno, H
Format: Article
Language:English
Subjects:
Agricultural runoff
Agricultural wastes
Anaerobic digestion
Anaerobic treatment
Animal wastes
Bacteria, Anaerobic - genetics
Bacteria, Anaerobic - physiology
Biodiversity
Biogas
Bioreactors
Cloning
Cluster Analysis
Digesters
DNA Primers
Ecosystem biology
Garbage
Livestock
Methane
Methane - biosynthesis
Microbial activity
Microorganisms
Molecular biology
Phylogenetics
Phylogeny
Polymerase Chain Reaction
Population
Population Dynamics
Refuse
Refuse Disposal - methods
Reproducibility
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Sewage sludge
Sludge
Species Specificity
Taxonomy
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Summary:The diversity of microbial communities in three full-scale thermophilic anaerobic digesters which treated garbage, sewage sludge and livestock wastes (hereafter called TGD, TSD and TLD, respectively) was investigated using 16S rDNA clone libraries in triplicate. The population dynamics of TGD were also studied. The purposes were to show the microbial diversity in each reactor and to suggest which key microbes in a thermophilic methane digester fed with garbage, including a check of reproducibility and the suggestion of an error range in this molecular biology method. 736 clones were identified, and the maximum error was estimated to be around +/-10% for the same OTU (operational taxonomic unit) and for most detected OTUs. The most frequently detected OTU shows a close relationship to Uncultured bacterium clone MBA08, Unidentified bacterium clone TUG22 and Uncultured archaeal symbiont PA204 in TGD, TSD and TLD, respectively. The microbial population dynamics in TGD were studied over a period of 90 days, and the occupying ratios of Bacillus infernus and Methanothermobacter wolfeii were shown to change with the change in VFA concentration. From the dynamic change and characteristics of the microbes, it is concluded that Bacillus infernus and Methanothermobacter wolfeii played an important role and were recommended as key microbes in TGD.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2007.320