Heterogeneous lack of expression of the tumour suppressor PTEN protein in human neoplastic tissues

PTEN, a tumour suppressor gene located at chromosome 10q23 and commonly mutated or deleted in a variety of tumours, encodes a dual-specific/phosphatidylinositol-3,4,5-triphosphate (PIP3) phosphatase. We report the generation of an anti-PTEN monoclonal antibody (MAb) that recognises an epitope at the...

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Bibliographic Details
Published in:European journal of cancer (1990) 2001, Vol.37 (1), p.114-121
Main Authors: Torres, J, Navarro, S, Roglá, I, Ripoll, F, Lluch, A, Garcı́a-Conde, J, Llombart-Bosch, A, Cervera, J, Pulido, R
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Biological and medical sciences
Cancer
Host-tumor relations. Immunology. Biological markers
Humans
Immunohistochemistry
Medical sciences
Mice
Mice, Inbred BALB C
Neoplasm Proteins - metabolism
Neoplasms - diagnosis
Phosphoric Monoester Hydrolases - metabolism
Protein phosphatases
PTEN
PTEN Phosphohydrolase
Tumor Cells, Cultured
Tumor Suppressor Proteins
Tumors
Tumour suppressor
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Summary:PTEN, a tumour suppressor gene located at chromosome 10q23 and commonly mutated or deleted in a variety of tumours, encodes a dual-specific/phosphatidylinositol-3,4,5-triphosphate (PIP3) phosphatase. We report the generation of an anti-PTEN monoclonal antibody (MAb) that recognises an epitope at the C-terminus of PTEN, and describe the heterogeneous lack of expression of the PTEN protein in human tumour tissues, as demonstrated by immunohistochemical methods. Our anti-PTEN MAb provides a useful tool for the study of PTEN protein expression in tumour samples, in the search for tumour prognostic molecular markers.
ISSN:0959-8049
1879-0852
DOI:10.1016/S0959-8049(00)00366-X