Simultaneous Detection and Genogroup-Screening Test for Norovirus Genogroups I and II from Fecal Specimens in Single Tube by Reverse Transcription-Loop-Mediated Isothermal Amplification Assay

We developed a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup‐specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT‐LAMP assay for the simultaneous...

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Bibliographic Details
Published in:Microbiology and immunology 2007-01, Vol.51 (5), p.547-550
Main Authors: Fukuda, Shinji, Sasaki, Yukie, Kuwayama, Masaru, Miyazaki, Kazuo
Format: Article
Language:English
Subjects:
Caliciviridae Infections - diagnosis
Caliciviridae Infections - epidemiology
Caliciviridae Infections - virology
Disease Outbreaks
DNA Primers - chemistry
DNA Primers - genetics
Feces - virology
Gastroenteritis - diagnosis
Gastroenteritis - epidemiology
Gastroenteritis - virology
genogrouping
Genotype
Humans
Norovirus
Norovirus - genetics
Norovirus - isolation & purification
NoV
Nucleic Acid Amplification Techniques - methods
Reverse Transcription
RNA, Viral - chemistry
RNA, Viral - genetics
RT-LAMP
Sensitivity and Specificity
simultaneous detection
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Summary:We developed a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) assay for the rapid detection of noroviruses (NoVs) in a genogroup‐specific manner in a previous study. In this study, to detect NoVs more easily and simply, we have developed an RT‐LAMP assay for the simultaneous detection of NoV genogroup I (GI) and II (GII) genomes in a single tube. The genogrouping was achieved by using fluorescence‐labeled primers, and the green and red colors for GI and GII, respectively, sometimes with yellow color for GI and GII mixture, were indicated on the agarose gel under UV light at 312 nm.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2007.tb03932.x