Regulatory Properties of Potato-Arabidopsis Hybrid ADP-Glucose Pyrophosphorylase

In higher plants, ADP-glucose pyrophosphorylase (ADPGlc-PPase) is a heterotetrameric enzyme comprised of two small and two large subunits. Potato-Arabidopsis hybrid ADPGlc-PPases were generated and their regulatory properties analyzed. We show that ADPGlc-PPase subunits from two different species ca...

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Bibliographic Details
Published in:Plant and cell physiology 2007-06, Vol.48 (6), p.875-880
Main Authors: Ventriglia, Tiziana, Ballicora, Miguel A., Crevillén, Pedro, Preiss, Jack, Romero, José M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - enzymology
Arabidopsis - genetics
Escherichia coli - genetics
Escherichia coli - metabolism
Glucose-1-Phosphate Adenylyltransferase - chemistry
Glucose-1-Phosphate Adenylyltransferase - genetics
Glucose-1-Phosphate Adenylyltransferase - metabolism
Molecular Sequence Data
Protein Subunits
Recombinant Proteins
Solanum tuberosum - enzymology
Solanum tuberosum - genetics
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Summary:In higher plants, ADP-glucose pyrophosphorylase (ADPGlc-PPase) is a heterotetrameric enzyme comprised of two small and two large subunits. Potato-Arabidopsis hybrid ADPGlc-PPases were generated and their regulatory properties analyzed. We show that ADPGlc-PPase subunits from two different species can interact, producing active enzymes with new regulatory properties. Depending on the subunit combinations, hybrid heterotetramers showed responses to allosteric effectors [3-phosphoglycerate (3-PGA) and Pi] in the micromolar or millimolar range. While hybrid potato small subunit (PSS) and the Arabidopsis large subunit APL1 showed an extremely sensitive response to 3-PGA and Pi, hybrid PSS/Arabidopsis APL2 was very insensitive to them. Intermediate responses were determined for other subunit combinations.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcm047