WRKY62 transcription factor acts downstream of cytosolic NPR1 and negatively regulates jasmonate-responsive gene expression

Cytosolic NPR1 has been shown to be essential for the salicylic acid (SA)-mediated suppression of jasmonic acid (JA)-responsive gene expression. However, factors downstream of NPR1 in the cross-talk between SA and JA signaling are unclear. Here we show that Arabidopsis WRKY62, a member of WRKY group...

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Bibliographic Details
Published in:Plant and cell physiology 2007-06, Vol.48 (6), p.833-842
Main Authors: Mao, P.(Peking Univ., Beijing (China)), Duan, M, Wei, C, Li, Y
Format: Article
Language:English
Subjects:
Acetates - pharmacology
Active Transport, Cell Nucleus
Amino Acid Sequence
ARABIDOPSIS
Arabidopsis - drug effects
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Basic-Leucine Zipper Transcription Factors - genetics
Basic-Leucine Zipper Transcription Factors - metabolism
Cyclopentanes - pharmacology
Cytosol - metabolism
GENE
Gene Expression Regulation, Plant - drug effects
GENES
JASMONATE
JASMONATES
Molecular Sequence Data
MUTANT
MUTANTES
MUTANTS
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Oxylipins
SALICILATOS
SALICYLATE
SALICYLATES
Salicylic Acid - pharmacology
Transcription Factors - chemistry
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Cytosolic NPR1 has been shown to be essential for the salicylic acid (SA)-mediated suppression of jasmonic acid (JA)-responsive gene expression. However, factors downstream of NPR1 in the cross-talk between SA and JA signaling are unclear. Here we show that Arabidopsis WRKY62, a member of WRKY group III transcription factors, was induced by methyl jasmonate (MeJA) and SA treatment. The presence of basal SA is required for the MeJA-induced WRKY62 expression, and both chemicals exhibit a synergistic effect on WRKY62 induction. In addition, upon treatment with an extremely low concentration of SA, cytosolic NPR1 controls the MeJA-induced expression of WRKY62. TGA transcription factors, which up-regulate SA-induced expression of WRKY62, are dispensable for the induction of WRKY62 in JA signaling. Genetic dissection of both wrky62 mutants and WRKY62-overexpressing plants indicated that WRKY62 down-regulates JA-responsive LOX2 and VSP2 expression. Our results demonstrate that WRKY62 acts downstream of cytosolic NPR1 and negatively regulates JA-responsive gene expression, suggesting that WRKY62 may be involved in the SA-mediated suppression of JA signaling.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcm058