Retinoic acid modulates the asialoglycoprotein receptor expression in cultured fetal rat hepatocytes

The influence of retinoic acid on the expression of a typical marker of hepatocyte differentiation, i.e. the asialoglycoprotein receptor, has been studied. Cultured hepatocytes, isolated from adult rats, a model of quiescent, mature cells and from 20-day-old fetuses, a model of proliferating and les...

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Bibliographic Details
Published in:Mechanisms of ageing and development 2001, Vol.122 (1), p.31-39
Main Authors: Falasca, Laura, Felici, Angelina, Massimi, Mara, Dini, Luciana, Conti Devirgiliis, Laura
Format: Article
Language:English
Subjects:
Adult and fetal rat
Animals
Antineoplastic Agents - pharmacology
ASGP-R
Asialoglycoprotein Receptor
Asialoglycoproteins - biosynthesis
Asialoglycoproteins - genetics
Biological and medical sciences
Blotting, Northern
Blotting, Western
Cells, Cultured - drug effects
Cultured hepatocytes
Female
Fundamental and applied biological sciences. Psychology
Hepatocytes - cytology
Hepatocytes - drug effects
Hepatocytes - metabolism
Liver - drug effects
Liver - embryology
Liver - metabolism
Liver. Bile. Biliary tracts
Male
Pregnancy
Rats
Rats, Wistar
Receptors, Cell Surface - biosynthesis
Receptors, Cell Surface - genetics
Retinoic acid
RNA, Messenger - genetics
RNA, Messenger - metabolism
Tretinoin - pharmacology
Vertebrates: digestive system
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Summary:The influence of retinoic acid on the expression of a typical marker of hepatocyte differentiation, i.e. the asialoglycoprotein receptor, has been studied. Cultured hepatocytes, isolated from adult rats, a model of quiescent, mature cells and from 20-day-old fetuses, a model of proliferating and less differentiated cells, were used. The asialoglycoprotein receptor expression appears to be affected by retinoic acid during prenatal life; both mRNA level and protein amount increased in fetal hepatocytes, but no modification has been found in adult cells, suggesting a regulative effect of retinoic acid during prenatal life, acting at transcriptional and/or translational level. Surprisingly, the receptor binding activity of adult hepatocytes is decreased after retinoic acid treatment, indicating a possible further modulation by this molecule on receptor activity at the post-translational level.
ISSN:0047-6374
1872-6216
DOI:10.1016/S0047-6374(00)00174-3