Functional characterization and genetic mapping of alk8

The novel type I TGFbeta family member receptor alk8 is expressed both maternally and zygotically. Functional characterization of alk8 was performed using microinjection studies of constitutively active (CA), kinase modified/dominant negative (DN), and truncated alk8 mRNAs. CA Alk8 expression produc...

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Bibliographic Details
Published in:Mechanisms of development 2001-02, Vol.100 (2), p.275-289
Main Authors: Payne, T L, Postlethwait, J H, Yelick, P C
Format: Article
Language:English
Subjects:
Activin Receptors
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins - biosynthesis
Chromosome Mapping
Conserved Sequence
Down-Regulation
Embryo, Nonmammalian - metabolism
Gene Expression Regulation, Developmental
Genes, Dominant
Glycoproteins - biosynthesis
Humans
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Mesoderm - metabolism
Models, Genetic
Neurons - metabolism
Phenotype
Phylogeny
Polymorphism, Single-Stranded Conformational
Protein Biosynthesis
Protein Structure, Tertiary
Protein-Serine-Threonine Kinases - biosynthesis
Protein-Serine-Threonine Kinases - genetics
RNA, Messenger - metabolism
Tissue Distribution
Transcription, Genetic
Up-Regulation
Xenopus Proteins
Zebrafish
Zebrafish Proteins
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Summary:The novel type I TGFbeta family member receptor alk8 is expressed both maternally and zygotically. Functional characterization of alk8 was performed using microinjection studies of constitutively active (CA), kinase modified/dominant negative (DN), and truncated alk8 mRNAs. CA Alk8 expression produces ventralized embryos while DN Alk8 expression results in dorsalized phenotypes. Truncated alk8 expressing embryos display a subtle dorsalized phenotype closely resembling that of the identified zebrafish dorsalized mutant, lost-a-fin (laf). Single-strand conformation polymorphism (SSCP) analysis was used to map alk8 to zebrafish LG02 in a region demonstrating significant conserved synteny to Hsa2, and which contains the human alk2 gene, ACVRI. Altogether, these functional, gene mapping and phylogenetic analyses suggest that alk8 may be the zebrafish orthologue to human ACVRI (alk2), and therefore extend previous studies of Alk2 conducted in Xenopus.
ISSN:0925-4773
DOI:10.1016/s0925-4773(00)00541-4