Comparative Structure, Proximal Promoter Elements, and Chromosome Location of the Human Eosinophil Major Basic Protein Genes

Human eosinophil major basic protein (MBP) is strongly implicated as a mediator of disease, especially bronchial asthma. We recently isolated a highly divergent human homologue of MBP (MBPH). Given human MBP's importance in disease and the restricted expression of it and human MBPH, we isolated...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2001-02, Vol.71 (3), p.271-281
Main Authors: Plager, Douglas A., Weiler, Deborah A., Loegering, David A., Johnson, William B., Haley, Linda, Eddy, Roger L., Shows, Thomas B., Gleich, Gerald J.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites
Biological and medical sciences
Blood Proteins - biosynthesis
Blood Proteins - genetics
CCAAT-Enhancer-Binding Proteins - metabolism
Centromere - ultrastructure
chromosome 11
Chromosome Mapping
Chromosomes, Human, Pair 11
Conserved Sequence
DNA, Complementary - metabolism
Eosinophil Granule Proteins
Eosinophil Major Basic Protein
Evolution, Molecular
Exons
Fundamental and applied biological sciences. Psychology
Gene Library
Genes. Genome
Humans
In Situ Hybridization, Fluorescence
Introns
major basic protein
Mice
Models, Genetic
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
PRG3 gene
Promoter Regions, Genetic
Ribonucleases
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Transcription, Genetic
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Summary:Human eosinophil major basic protein (MBP) is strongly implicated as a mediator of disease, especially bronchial asthma. We recently isolated a highly divergent human homologue of MBP (MBPH). Given human MBP's importance in disease and the restricted expression of it and human MBPH, we isolated the 4.6-kb human MBPH gene (HGMW-approved symbol PRG3). Comparisons among the human MBP (PRG2), human MBPH, and murine MBP-1 (mMBP-1; Prg2) genes suggest that the human MBP and mMBP-1 genes are more closely related than either is to the human MBPH gene. Proximal promoters of these three genes show conservation of potential binding sites for IK2 and STAT and of a known GATA site. However, a known C/EBP site is altered in the human MBPH gene's proximal promoter. The human MBP and MBPH genes localized to chromosome 11 in the centromere to 11q12 region. Thus, the human MBP and MBPH genes have diverged considerably, probably following a gene duplication event. Furthermore, the identified conserved and distinct proximal promoter elements likely contribute to the eosinophil-restricted and relatively reduced transcription of the human MBPH gene.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.2000.6391