Proteome analysis of human androgen-independent prostate cancer cell lines: Variable metastatic potentials correlated with vimentin expression

• Published in: Proteomics (Weinheim) 2007-06, Vol.7 (12), p.1973-1983
• Main Authors: Wu, Mingfu, Bai, Xiangyang, Xu, Gang, Wei, Juncheng, Zhu, Tao, Zhang, Yongtao, Li, Qiong, Liu, Ping, Song, Anping, Zhao, Liangpin, Gang, Chen, Han, Zhiqiang, Wang, Shixuan, Zhou, Jianfeng, Lu, Yunpin, Ma, Ding
• Format: Article
• Language: English
• Subjects: 2-DE; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Androgen-independent prostate cancer; Androgens - physiology; Animals; Biological and medical sciences; Cell Line, Tumor; Fundamental and applied biological sciences. Psychology; Gynecology. Andrology. Obstetrics; Humans; Lung Neoplasms - secondary; Lymph Nodes - pathology; Male; Male genital diseases; Medical sciences; Metastasis; Mice; Mice, Inbred BALB C; Miscellaneous; Molecular Sequence Data; Neoplasm Metastasis; Nephrology. Urinary tract diseases; Prostatic Neoplasms - metabolism; Proteins; Proteome - metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tumors; Tumors of the urinary system; Urinary tract. Prostate gland; Vimentin; Vimentin - metabolism
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200600643

To better understand the molecular mechanisms of prostate cancer (PCA) dissemination and to develop new anti‐metastasis therapies, key regulatory molecules involved in PCA metastasis were identified in two human androgen‐independent PCA cell lines, highly metastatic 1E8‐H and lowly metastatic 2B4‐L cells. Through 2‐DE and MS analyses, 12 proteins with different expression levels in the two cell lines were identified. The following proteins were found to be significantly up‐regulated in 1E8‐H cells compared with 2B4‐L cells: gp96 precursor, calreticulin precursor, vimentin (VIM), Hsp90α, peroxiredoxin 2, HNRPH1, ezrin, T‐complex protein 1, alpha subunit, and hypothetical protein mln2339. In contrast, heart L‐lactate dehydrogenase H chain, annexin I, and protein disulfide isomerase were notably down‐regulated in 1E8‐H cells compared with 2B4‐L cells. To our knowledge, this study is the first to demonstrate that up‐regulation of VIM expression positively correlates with the invasion and metastasis of androgen‐independent PCA.