Bacillus subtilis cells immobilised in PVA-cryogels

Bacillus subtilis viable cells were immobilised in PVA-cryogel beads using the ‘freezing-thawing’ method in a two-phase (water–oil) system. Conditions providing both high thermal and mechanical stability and suitable porosity of the carrier were optimised. For monitoring and analysis of changes insi...

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Bibliographic Details
Published in:Biomolecular engineering 2001, Vol.17 (2), p.55-63
Main Authors: Szczęsna-Antczak, M, Galas, E
Format: Article
Language:English
Subjects:
Albumins - chemistry
algin
Alginates - chemistry
azoalbumin
azocasein
B. subtilis
Bacillus subtilis
Bacillus subtilis - cytology
Bacillus subtilis - physiology
Caseins - chemistry
cellulose
Cellulose - chemistry
Cryopreservation - methods
Freezing
Gels - chemistry
Glucuronic Acid
hemoglobin
Hemoglobins - chemistry
Hexuronic Acids
Image analysis
Immobilisation
Models, Statistical
Polyvinyl Alcohol - chemistry
Porosity
Proteinases
PVA-cryogels
sodium alginate
Temperature
Time Factors
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Summary:Bacillus subtilis viable cells were immobilised in PVA-cryogel beads using the ‘freezing-thawing’ method in a two-phase (water–oil) system. Conditions providing both high thermal and mechanical stability and suitable porosity of the carrier were optimised. For monitoring and analysis of changes inside the biocatalyst beads, and for determination of diffusive properties of the carrier, an image analysis was applied. It was revealed that bacterial spores, sodium alginate and bacterial cellulose accelerated hardening of the cryogels and modified their porosity. Proteins (haemoglobin, azoalbumin, azocasein) penetrated beads of the cryogel.
ISSN:1389-0344
1878-559X
DOI:10.1016/S1389-0344(00)00065-4