Temporally and spatially regulated expression of a candidate G‐protein‐coupled receptor during cerebral cortical development

Genes expressed in layer‐specific patterns in the mammalian cerebral cortex may play a role in specifying the identity of different cortical layers. Using PCR‐differential display, we identified a cDNA that encodes rCNL3, a gene cloned previously by sequence homology to G‐protein‐coupled receptors....

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Bibliographic Details
Published in:Journal of neurobiology 2001-02, Vol.46 (3), p.167-177
Main Authors: Chenn, Anjen, Levin, Margaret E., McConnell, Susan K.
Format: Article
Language:English
Subjects:
Age Factors
Animals
Animals, Newborn
Base Sequence
Body Patterning - physiology
Cell Differentiation - physiology
cell fate
cerebral cortex
Cerebral Cortex - cytology
Cerebral Cortex - embryology
Cerebral Cortex - growth & development
Cloning, Organism - methods
Darkness
Fetus
Gene Expression Regulation, Developmental - physiology
GTP-Binding Proteins - metabolism
G‐protein‐coupled receptor
layers
Molecular Sequence Data
Neurons - cytology
Neurons - metabolism
PCR‐differential display
Rats
Receptors, Cell Surface - genetics
Receptors, G-Protein-Coupled
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Stem Cells - cytology
Stem Cells - metabolism
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Summary:Genes expressed in layer‐specific patterns in the mammalian cerebral cortex may play a role in specifying the identity of different cortical layers. Using PCR‐differential display, we identified a cDNA that encodes rCNL3, a gene cloned previously by sequence homology to G‐protein‐coupled receptors. rCNL3 is expressed predominantly in layers 2–4 of the young rat cortex and in the developing and adult striatum. Cortical expression of rCNL3 begins postnatally at P3 and continues at high levels until around P15, while striatal expression begins at E20 and continues through adulthood. rCNL3 expression is not detectable in the ventricular zone precursors that generate the neurons of layers 2–4. The expression pattern of rCNL3 in the developing cortex suggests that rCNL3 is not involved in the initial specification of laminar fate, but rather may be involved with later differentiation events within the superficial cortical layers. © 2001 John Wiley & Sons, Inc. J Neurobiol 46: 167–177, 2001
ISSN:0022-3034
1097-4695
DOI:10.1002/1097-4695(20010215)46:3<167::AID-NEU1000>3.0.CO;2-J