A Common Deletion at Chromosomal Region 17q21 in Sporadic Prostate Tumors Distal to BRCA1

Prostate cancer is the most common cancer in males in the United States, yet the etiology of this disease is still poorly understood. In previous work from our laboratory, one or more deleted regions were found in prostate tumors distal to the breast and ovarian cancer susceptibility gene (BRCA1) on...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 2001-02, Vol.71 (3), p.324-329
Main Authors: Dai, Qiang, Deubler, Debra A., Maxwell, Teresa M., Zhu, Xiao Lin, Cui, Jiang, Rohr, L.Ralph, Stephenson, Robert A., Brothman, Arthur R.
Format: Article
Language:English
Subjects:
Biological and medical sciences
BRCA1 gene
chromosome 17
Chromosome aberrations
Chromosome Deletion
Chromosomes, Human, Pair 17
Contig Mapping
Databases, Factual
DNA Mutational Analysis
Expressed Sequence Tags
Gene Deletion
Genes, BRCA1 - genetics
Genetic Markers
Humans
In Situ Hybridization, Fluorescence
Male
Medical genetics
Medical sciences
Models, Genetic
Molecular Sequence Data
Physical Chromosome Mapping
Polymerase Chain Reaction
prostate carcinoma
Prostatic Neoplasms - genetics
Sequence Tagged Sites
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Summary:Prostate cancer is the most common cancer in males in the United States, yet the etiology of this disease is still poorly understood. In previous work from our laboratory, one or more deleted regions were found in prostate tumors distal to the breast and ovarian cancer susceptibility gene (BRCA1) on chromosome 17. This suggested that genes at 17q21 may play a pivotal role in prostate cancer progression, and there may be new tumor suppressor genes at this locus. We now present a physical map built with P1, P1 artificial chromosome, and bacterial artificial chromosome clones encompassing a DNA sequence anchored by multiple STS markers. The analysis of prostate tumors indicated an 85-kb novel commonly deleted interval flanked by D17S1184–D17S183–D17S1203–D17S1860, which is at least 470 kb distal to the BRCA1 gene. Fifty-four of 126 prostrate cancer cases (43%) showed a deletion by a direct FISH technique using P1 probes in this region. Searching with clone end sequences in the sequence database BLAST, the deleted clone covered genomic DNA sequence that contained upstream binding factor (UBF), EPB3 genes, SHCL1, ASB-4-like sequence, and acidic protein-like sequence. PCR for the ESTs confirmed that these genes or ESTs are within the deletion region. Our results will be helpful for finding candidate tumor suppressor genes in prostate cancer.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.2000.6436