Identification and expression of the motilin precursor in the guinea pig

• Published in: FEBS letters 2001-02, Vol.490 (1), p.7-10
• Main Authors: Xu, Luo, Depoortere, Inge, Tang, Ming, Peeters, Theo L.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Brain - metabolism; Cloning, Molecular; Distribution; DNA Primers - metabolism; DNA, Complementary - metabolism; Duodenum - metabolism; Guinea Pigs; Homology; Humans; Molecular Sequence Data; Motilin; Motilin - biosynthesis; Motilin - chemistry; Motilin - genetics; Mucous Membrane - metabolism; Polymerase Chain Reaction; Precursor; Protein Precursors - chemistry; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Thyroid; Thyroid Gland - metabolism; Tissue Distribution
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(01)02125-1

Motilin has never been isolated from rodents, the most frequently used laboratory animals, despite several attempts. We have isolated and sequenced the motilin precursor from duodenal mucosa of guinea pig (GenBank accession number AF323752) and studied its expression in several tissues. The percent homology with human motilin is the lowest yet observed due to several unique substitutions in the C-terminal end. As expected, the precursor was present in the gut mucosa with the exception of the gastric corpus. It was also present in medulla oblongata, nucleus of the solitary tract, hypophysis, spinal cord, hypothalamus, and cerebellum but not in the cerebral cortex. For the first time we demonstrated motilin expression in the thyroid.