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Human Peripheral Blood Cells Differentially Recognize and Respond to Two Distinct CpG Motifs
Oligodeoxynucleotides (ODN) that contain unmethylated CpG dinucleotides trigger a strong innate immune response in vertebrates. CpG ODN show promise as vaccine adjuvants, anti-allergens, and immunoprotective agents in animal models. Their transition to clinical use requires the identification of mot...
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| Published in: | The Journal of immunology (1950) 2001-02, Vol.166 (4), p.2372-2377 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c475t-69f5eee6677f9f07388423a1bb2fe4a981c9b343828c3c1b0e45d1ada0ce6caa3 |
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| cites | cdi_FETCH-LOGICAL-c475t-69f5eee6677f9f07388423a1bb2fe4a981c9b343828c3c1b0e45d1ada0ce6caa3 |
| container_end_page | 2377 |
| container_issue | 4 |
| container_start_page | 2372 |
| container_title | The Journal of immunology (1950) |
| container_volume | 166 |
| creator | Verthelyi, Daniela Ishii, Ken J Gursel, Mayda Takeshita, Fumihiko Klinman, Dennis M |
| description | Oligodeoxynucleotides (ODN) that contain unmethylated CpG dinucleotides trigger a strong innate immune response in vertebrates. CpG ODN show promise as vaccine adjuvants, anti-allergens, and immunoprotective agents in animal models. Their transition to clinical use requires the identification of motifs that are optimally stimulatory in humans. Analysis of hundreds of novel ODN resulted in the identification and characterization of two structurally distinct "clusters" of immunostimulatory CpG ODN. One cluster ("D") preferentially stimulates IFN-gamma production by NK cells, whereas the other ("K") stimulates cell proliferation and the production of IL-6 and IgM by monocytes and B cells. The distinct immunostimulatory properties of K and D ODN can improve the design of CpG-based products to achieve specific therapeutic goals. |
| doi_str_mv | 10.4049/jimmunol.166.4.2372 |
| format | article |
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| ispartof | The Journal of immunology (1950), 2001-02, Vol.166 (4), p.2372-2377 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70635493 |
| source | EZB Electronic Journals Library |
| subjects | Adjuvants, Immunologic - chemistry Adjuvants, Immunologic - pharmacology B-Lymphocytes - immunology B-Lymphocytes - metabolism Cell Division - immunology Cell Line CpG Islands - immunology Dendritic Cells - immunology Dendritic Cells - metabolism Humans Immunoglobulin M - biosynthesis Interferon-gamma - metabolism Interleukin-6 - metabolism Killer Cells, Natural - immunology Killer Cells, Natural - metabolism Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Lymphocyte Activation - immunology Monocytes - immunology Monocytes - metabolism Oligodeoxynucleotides Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - pharmacology Tumor Cells, Cultured |
| title | Human Peripheral Blood Cells Differentially Recognize and Respond to Two Distinct CpG Motifs |
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