BglII and MunI: what a difference a base makes

Restriction endonucleases are resilient to alterations in their DNA-binding specificities. Structures of the BglII and MunI endonucleases bound to their palindromic DNA sites, which differ by only their outer base pairs from the recognition sequences of BamHI and EcoRI, respectively, have recently b...

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Bibliographic Details
Published in:Current Opinion in Structural Biology 2001-02, Vol.11 (1), p.14-18
Main Authors: Lukacs, Christine M, Aggarwal, Aneel K
Format: Article
Language:English
Subjects:
Bacterial Proteins
BamHl
BgIII
deoxyribonuclease BamIII
deoxyribonuclease BglII
deoxyribonuclease MunI
Deoxyribonucleases, Type II Site-Specific - chemistry
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA
DNA-Binding Proteins - chemistry
DNA-Binding Proteins - metabolism
EcoRI
enzyme
Models, Molecular
Munl
recognition
Restriction endonuclease
specificity
Substrate Specificity
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Summary:Restriction endonucleases are resilient to alterations in their DNA-binding specificities. Structures of the BglII and MunI endonucleases bound to their palindromic DNA sites, which differ by only their outer base pairs from the recognition sequences of BamHI and EcoRI, respectively, have recently been determined. A comparison of these complexes reveals surprising differences and similarities in structure, and provides a basis for understanding the immutability of restriction endonucleases.
ISSN:0959-440X
1879-033X
DOI:10.1016/S0959-440X(00)00174-3