Enoate reductases of Clostridia. Cloning, sequencing, and expression

The enr genes specifying enoate reductases of Clostridium tyrobutyricum and Clostridium thermoaceticum were cloned and sequenced. Sequence comparison shows that enoate reductases are similar to a family of flavoproteins comprising 2,4-dienoyl-coenzyme A reductase from Escherichia coli and old yellow...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 2001-02, Vol.276 (8), p.5779-5787
Main Authors: Rohdich, F, Wiese, A, Feicht, R, Simon, H, Bacher, A
Format: Article
Language:English
Subjects:
2,4-Dienoyl-CoA reductase
2,4-dienoyl-coenzyme A reductase
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Clostridium - enzymology
Clostridium thermoaceticum
enr gene
Escherichia coli
Fatty Acid Desaturases - genetics
Genes, Bacterial
Molecular Sequence Data
Oxidoreductases Acting on CH-CH Group Donors
Recombinant Proteins - biosynthesis
Sequence Analysis, DNA
Sequence Analysis, Protein
Sequence Homology, Amino Acid
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The enr genes specifying enoate reductases of Clostridium tyrobutyricum and Clostridium thermoaceticum were cloned and sequenced. Sequence comparison shows that enoate reductases are similar to a family of flavoproteins comprising 2,4-dienoyl-coenzyme A reductase from Escherichia coli and old yellow enzyme from yeast. The C. thermoaceticum enr gene product was expressed in recombinant Escherichia coli cells growing under anaerobic conditions. The recombinant enzyme was purified and characterized.
ISSN:0021-9258
DOI:10.1074/jbc.M008656200