Determination of ethyl- p-hydroxybenzoate in sow pancreatic juice by reversed-phase high-performance liquid chromatography

We have developed a high-performance liquid chromatographic–UV–Vis–diode-array detection (HPLC–DAD) method for the determination of ethyl- p-hydroxybenzoate, a hydrolytic degradation product of the synthetic protease inhibitor, gabexate-mesilate ethyl- p-(6-guanidinohexanoyloxy) benzoate methanesulf...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-02, Vol.751 (2), p.365-369
Main Authors: Di Giovannandrea, R., Diana, L., Fiori, M., Ferretti, E., Foglietta, G., Caronna, R., Severini, G.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid - methods
Ethyl- p-hydroxybenzoate
Female
General pharmacology
Medical sciences
Pancreatic Juice - chemistry
Parabens - analysis
Pharmacology. Drug treatments
Reproducibility of Results
Spectrophotometry, Ultraviolet
Swine
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Summary:We have developed a high-performance liquid chromatographic–UV–Vis–diode-array detection (HPLC–DAD) method for the determination of ethyl- p-hydroxybenzoate, a hydrolytic degradation product of the synthetic protease inhibitor, gabexate-mesilate ethyl- p-(6-guanidinohexanoyloxy) benzoate methanesulfonate (GM) (FOY) in sow pancreatic juice. Methyl- p-hydroxybenzoate ( I) was used as the internal standard. The pancreatic juice was deproteinised by acetonitrile and the analytes were chromatographed on a reversed-phase C 18 LC column using the gradient elution method. The mobile phase consisted of a solution of 0.017 M orthophosphoric acid and another solution of acetonitrile–water (80:20, v/v). The wavelength of detection was 237 nm. The limit of quantification of the method was 0.20 μ M at a 9:1 signal-to-noise ratio. The overall intra- and inter-day accuracy (relative error, RE) ranged from 14.2 to 8.3% and from 13.3 to 9.8, respectively. The overall intra- and inter-day precision (relative standard deviation, RSD) ranged from 7.6 to 2.62% and from 6.7 to 3.1%, respectively. The method proved to be sensitive, specific, accurate and precise and was successfully used to determine the ethyl- p-hydroxybenzoate ( II) in sow pancreatic juice.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00477-1