Disruption and functional analysis of six ORFs of chromosome IV: YDL103c (QRI1), YDL105w (QRI2), YDL112w (TRM3), YDL113c, YDL116w (NUP84) and YDL167c (NRP1)

• Published in: Yeast (Chichester, England) England), 2001-02, Vol.18 (3), p.273-282
• Main Authors: Reynaud, Anne, Facca, Céline, Sor, Frédéric, Faye, Gérard
• Format: Article
• Language: English
• Subjects: chromosome IV; Chromosomes, Fungal - chemistry; Chromosomes, Fungal - genetics; DNA Primers - chemistry; DNA, Fungal - chemistry; functional analysis; Gene Deletion; gene disruption; Genes, Fungal - genetics; NRP1 gene; NUP84 gene; Open Reading Frames - genetics; Phenotype; Polymerase Chain Reaction; QRI1 gene; QRI2 gene; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - genetics; TRM3 gene; YDL103c gene; YDL105w gene; YDL112w gene; YDL113c gene; YDL116w gene; YDL167c gene
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/1097-0061(200102)18:3<273::AID-YEA665>3.0.CO;2-B

We have disrupted six ORFs (YDL103c, YDL105w, YDL112w, YDL113c, YDL116w and YDL167c) located on the left arm of chromosome IV. Except for YDL112w, the short flanking homology strategy was used to construct disruption cassettes using the KanMX4 marker. For YDL112w, a disruption cassette including the LEU2 gene was made. YDL103c and YDL105w are essential genes for vegetative growth. Disruption of YDL112w, YDL113c and YDL167c does not result in any detectable phenotype with the tests we used, while disruption of YDL116w confers slow growth, cryosensitivity and thermosensitivity, and the disrupted diploid homozygotes for the disruption failed to sporulate. Copyright © 2000 John Wiley & Sons, Ltd.