Replicative stress after allogeneic bone marrow transplantation: changes in cycling of CD34+CD90+ and CD34+CD90− hematopoietic progenitors

• Published in: Blood 2001-03, Vol.97 (6), p.1876-1878
• Main Authors: Thornley, Ian, Sutherland, D. Robert, Nayar, Rakash, Sung, Lillian, Freedman, Melvin H., Messner, Hans A.
• Format: Article
• Language: English
• Subjects: Adult; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Antigens, CD34 - physiology; Biological and medical sciences; Bone Marrow Cells - cytology; Bone Marrow Cells - immunology; Bone Marrow Transplantation - physiology; Bone marrow, stem cells transplantation. Graft versus host reaction; Cell Cycle - physiology; Cell Division - immunology; Cell Division - physiology; Flow Cytometry; Follow-Up Studies; Hematologic Neoplasms - therapy; Hematopoiesis; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - immunology; Humans; Medical sciences; Middle Aged; Thy-1 Antigens - physiology; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation, Homologous - physiology
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V97.6.1876

To further characterize hematopoietic “replicative stress” induced by bone marrow transplantation (BMT), the cell-cycle status of CD90+/− subsets of marrow CD34+ cells obtained 2 to 6 months after transplantation from 11 fully chimeric recipients was examined. Cycling profiles, derived by flow cytometry after staining with Hoechst 33342 and pyronin Y, were compared with those of 14 healthy marrow donors. Primitive CD34+CD90+cells represented a smaller proportion of CD34+ cells in recipients (10% ± 4% versus 19.6% ± 5.3% in donors;P < .0001) and were more mitotically active, with the proportion of cells in S/G2/M nearly 4-fold higher than in donors (15.6% ± 3% and 4.4% ± 1.6%, respectively;P < .0001). By comparison, there was a modest increase in the proportion of CD34+CD90−progenitors in S/G2/M after BMT (10.9% ± 1% vs 9.6% ± 2% in donors; P = .04). Replicative stress after BMT is borne predominantly by cells in a diminished CD34+CD90+ population.