NK1.1+ Cells and Murine Cytomegalovirus Infection: What Happens In Situ?

NK cells mediate early host defense against viral infection. In murine CMV (MCMV) infection NK cells play a critical role in controlling viral replication in target organs, such as spleen and liver. Until now it has not been possible to directly examine the role of NK cells in MCMV-induced inflammat...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2001-02, Vol.166 (3), p.1796-1802
Main Authors: Andrews, Daniel M, Farrell, Helen E, Densley, Eloise H, Scalzo, Anthony A, Shellam, Geoffrey R, Degli-Esposti, Mariapia A
Format: Article
Language:English
Subjects:
Animals
Antigens - analysis
Antigens - biosynthesis
Antigens, Ly
Antigens, Surface
Biomarkers - analysis
Fluorescent Antibody Technique, Indirect
Herpesviridae Infections - immunology
Herpesviridae Infections - metabolism
Herpesviridae Infections - pathology
Killer Cells, Natural - chemistry
Killer Cells, Natural - immunology
Killer Cells, Natural - virology
Lectins, C-Type
Liver - chemistry
Liver - immunology
Liver - pathology
Liver - virology
Lung - chemistry
Lung - immunology
Lung - pathology
Lung - virology
Mice
Mice, Congenic
Mice, Inbred BALB C
Mice, Inbred C57BL
Muromegalovirus - immunology
NK Cell Lectin-Like Receptor Subfamily B
Protein Biosynthesis
Proteins - analysis
Spleen - chemistry
Spleen - immunology
Spleen - pathology
Spleen - virology
Viral Plaque Assay
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Summary:NK cells mediate early host defense against viral infection. In murine CMV (MCMV) infection NK cells play a critical role in controlling viral replication in target organs, such as spleen and liver. Until now it has not been possible to directly examine the role of NK cells in MCMV-induced inflammation in situ due to the inability to stain specifically for NK cells in infected tissues. In this study, we describe a method of in vivo fixation, resulting in the first identification of NK cells in situ using NK1.1 as the marker. Using this method, we characterize the NK1.1(+) cellular component of the inflammatory response to wild-type MCMV in the spleen, liver, and lung of genetically susceptible and resistant mice following i.p. infection. This study provides the first in situ description of the cellular response mediated specifically by NK cells following MCMV infection.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.166.3.1796