Partial purification of a novel insect antidiuretic factor from the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), which acts on Malpighian tubules

In this study, we report for the first time the presence of an antidiuretic factor in the head of the Colorado potato beetle ( Leptinotarsa decemlineata (Say)) which acts directly on Malpighian tubules. Biologically active fractions were isolated from the head and separated using molecular weight fi...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2001-03, Vol.31 (4), p.339-347
Main Authors: Lavigne, Carole, Embleton, Jean, Audy, Patrice, King, Russell R, Pelletier, Yvan
Format: Article
Language:English
Subjects:
Agriculture
Animals
Antidiuretic factor
Chrysomelidae
Coleoptera - chemistry
Diuresis - drug effects
Fluid secretion
HPLC purification
Insect Control
Leptinotarsa decemlineata
Malpighian tubule
Malpighian Tubules - drug effects
Peptides - isolation & purification
Peptides - pharmacology
Renal Agents - isolation & purification
Renal Agents - pharmacology
Water - metabolism
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Summary:In this study, we report for the first time the presence of an antidiuretic factor in the head of the Colorado potato beetle ( Leptinotarsa decemlineata (Say)) which acts directly on Malpighian tubules. Biologically active fractions were isolated from the head and separated using molecular weight filtration and high-performance liquid chromatography (HPLC). The resulting fractions were tested for their antidiuretic activity on single isolated Malpighian tubules. Antidiuretic activity was found in the 25% acetonitrile Sep-Pak fraction and the Cn-2 (3000–10,000 MW) and Cn-3 (
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(00)00126-0