Versican accumulation in human prostatic fibroblast cultures is enhanced by prostate cancer cell-derived transforming growth factor beta1

We have previously demonstrated that peritumoral stromal matrix derived from prostate cancer patients who relapse after radical surgery contains elevated levels of versican. The purpose of this study was to determine whether prostate cancer cells control stromal cell secretion of versican. Serum-fre...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2001-02, Vol.61 (3), p.926-930
Main Authors: Sakko, A J, Ricciardelli, C, Mayne, K, Tilley, W D, Lebaron, R G, Horsfall, D J
Format: Article
Language:English
Subjects:
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Cell Communication - physiology
Cell Division
Cells, Cultured
Chondroitin Sulfate Proteoglycans - biosynthesis
Chondroitin Sulfate Proteoglycans - genetics
Chondroitin Sulfate Proteoglycans - secretion
Coculture Techniques
Culture Media, Conditioned
Fibroblasts - cytology
Fibroblasts - metabolism
Fibroblasts - secretion
Humans
Immunoblotting
Lectins, C-Type
Male
Prostate - cytology
Prostate - metabolism
Prostate - secretion
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Stromal Cells - metabolism
Stromal Cells - secretion
Transforming Growth Factor beta - biosynthesis
Transforming Growth Factor beta - pharmacology
Transforming Growth Factor beta1
Versicans
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Summary:We have previously demonstrated that peritumoral stromal matrix derived from prostate cancer patients who relapse after radical surgery contains elevated levels of versican. The purpose of this study was to determine whether prostate cancer cells control stromal cell secretion of versican. Serum-free conditioned medium from three prostate adenocarcinoma cell lines, LNCaP, PC3, and DU145, was added to cultures of fibroblasts established from prostatic tissue of patients with benign prostatic hyperplasia, and the medium was harvested at 24, 48, and 72 h. Immunoblotting with an antiversican core protein antibody revealed that prostatic fibroblast medium harvested at 72 h contained increased levels of versican after treatment with either LNCaP-, PC3- or DU145-conditioned medium (2.5-, 4.5-, and 5-fold, respectively) compared with control cultures. This increase in versican in the culture medium was not observed after coincubation with transforming growth factor beta1-neutralizing antisera. The results of this study suggest that prostate tumor cells induce host stromal cells to secrete increased versican levels via a paracrine mechanism mediated by transforming growth factor beta1.
ISSN:0008-5472