Versican accumulation in human prostatic fibroblast cultures is enhanced by prostate cancer cell-derived transforming growth factor beta1

• Published in: Cancer research (Chicago, Ill.) Ill.), 2001-02, Vol.61 (3), p.926-930
• Main Authors: Sakko, A J, Ricciardelli, C, Mayne, K, Tilley, W D, Lebaron, R G, Horsfall, D J
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Cell Communication - physiology; Cell Division; Cells, Cultured; Chondroitin Sulfate Proteoglycans - biosynthesis; Chondroitin Sulfate Proteoglycans - genetics; Chondroitin Sulfate Proteoglycans - secretion; Coculture Techniques; Culture Media, Conditioned; Fibroblasts - cytology; Fibroblasts - metabolism; Fibroblasts - secretion; Humans; Immunoblotting; Lectins, C-Type; Male; Prostate - cytology; Prostate - metabolism; Prostate - secretion; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Stromal Cells - metabolism; Stromal Cells - secretion; Transforming Growth Factor beta - biosynthesis; Transforming Growth Factor beta - pharmacology; Transforming Growth Factor beta1; Versicans
• ISSN: 0008-5472

We have previously demonstrated that peritumoral stromal matrix derived from prostate cancer patients who relapse after radical surgery contains elevated levels of versican. The purpose of this study was to determine whether prostate cancer cells control stromal cell secretion of versican. Serum-free conditioned medium from three prostate adenocarcinoma cell lines, LNCaP, PC3, and DU145, was added to cultures of fibroblasts established from prostatic tissue of patients with benign prostatic hyperplasia, and the medium was harvested at 24, 48, and 72 h. Immunoblotting with an antiversican core protein antibody revealed that prostatic fibroblast medium harvested at 72 h contained increased levels of versican after treatment with either LNCaP-, PC3- or DU145-conditioned medium (2.5-, 4.5-, and 5-fold, respectively) compared with control cultures. This increase in versican in the culture medium was not observed after coincubation with transforming growth factor beta1-neutralizing antisera. The results of this study suggest that prostate tumor cells induce host stromal cells to secrete increased versican levels via a paracrine mechanism mediated by transforming growth factor beta1.