Altered Cardiac Sarcoplasmic Reticulum Function of Intact Myocytes of Rat Ventricle During Metabolic Inhibition

ABSTRACT —Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca and the perforated patch-clamp technique to measure SR Ca content. Metaboli...

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Bibliographic Details
Published in:Circulation research 2001-02, Vol.88 (2), p.181-187
Main Authors: Overend, C L, Eisner, D A, O’Neill, S C
Format: Article
Language:English
Subjects:
Aniline Compounds
Animals
Biological and medical sciences
Cadmium - pharmacology
Calcium - metabolism
Calcium Channel Blockers - pharmacology
Calcium Channels, L-Type - metabolism
Calcium Signaling - physiology
Calcium-Transporting ATPases - antagonists & inhibitors
Calcium-Transporting ATPases - metabolism
Cardiology. Vascular system
Cells, Cultured
Coronary heart disease
Cyanides - pharmacology
Deoxyglucose - pharmacology
Fluorescent Dyes
Heart
Heart Ventricles - cytology
Heart Ventricles - metabolism
Intracellular Fluid - metabolism
Medical sciences
Microscopy, Confocal
Myocardium - cytology
Myocardium - metabolism
Patch-Clamp Techniques
Rats
Ryanodine Receptor Calcium Release Channel - metabolism
Sarcoplasmic Reticulum - metabolism
Xanthenes
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Summary:ABSTRACT —Changes in the behavior of the sarcoplasmic reticulum (SR) in rat ventricular myocytes were investigated under conditions of metabolic inhibition using laser-scanning confocal microscopy to measure intracellular Ca and the perforated patch-clamp technique to measure SR Ca content. Metabolic inhibition had several effects on SR function, including reduced frequency of spontaneous releases of Ca (sparks and waves of Ca-induced Ca release), increased SR Ca content (79.4±5.7 to 115.2±6.6 μmol/L cell volume [mean±SEM;P
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.88.2.181