Developmental profile of the changes in the prothoracicotropic hormone titer in hemolymph of the silkworm Bombyx mori: correlation with ecdysteroid secretion

A very sensitive time-resolved fluoroimmunoassay for the prothoracicotropic hormone (PTTH) of the silkworm Bombyx mori has been established. The lower limit of detection in this assay was 0.1 pg. With this assay method, the amounts of PTTH in the central nervous system and hemolymph were quantified....

Full description

Saved in:
Bibliographic Details
Published in:Insect biochemistry and molecular biology 2001-03, Vol.31 (4), p.349-358
Main Authors: Mizoguchi, Akira, Ohashi, Yasutaka, Hosoda, Kazutaka, Ishibashi, Jun, Kataoka, Hiroshi
Format: Article
Language:English
Subjects:
Animals
Bombyx - chemistry
Bombyx - metabolism
Bombyx mori
Brain Chemistry
Ecdysteroid
Ecdysteroids
Fluoroimmunoassay - methods
Hemolymph
Hemolymph - chemistry
Hemolymph - metabolism
Hormone secretion
Insect Hormones - analysis
Insect Hormones - metabolism
Larva - metabolism
Metamorphosis, Biological - physiology
Oligopeptides - analysis
Oligopeptides - metabolism
Prothoracic gland
Prothoracicotropic hormone
Prothoracicotropic hormone (PTTH)
Pupa - metabolism
Steroids - metabolism
Time-resolved fluoroimmunoassay
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A very sensitive time-resolved fluoroimmunoassay for the prothoracicotropic hormone (PTTH) of the silkworm Bombyx mori has been established. The lower limit of detection in this assay was 0.1 pg. With this assay method, the amounts of PTTH in the central nervous system and hemolymph were quantified. PTTH was detected only in the brain within the central nervous system, and, in the fifth instar, its content in the brain increased gradually with larval growth and decreased rapidly after the beginning of wandering. A substantial amount of PTTH was also found in the retrocerebral complex of day-3 fifth instar larvae, accounting for 28% of total PTTH. The PTTH titer in hemolymph changed dramatically during Bombyx development, with a small peak in the middle of the fourth instar, medium-sized peaks at the wandering and prepupal stages in the fifth instar, and a large prolonged peak during early pupal–adult development. The changes were overall closely correlated with those in hemolymph ecdysteroid titer. However, some unexpected aspects of PTTH dynamics in hemolymph have also been disclosed. Based on these observations, the significance of PTTH secretion in the control of insect development is discussed.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(00)00127-2