Peripheral nerve regeneration by the in vitro differentiated-human bone marrow stromal cells with Schwann cell property

We examined the availability of human bone marrow stromal cells (MSCs) as a source of transplantation therapy in nerve injury. Human MSCs were subjected to a series of treatments with a reducing agent, retinoic acid and a combination of trophic factors. Morphologically and immunocytochemically, such...

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Published in:Biochemical and biophysical research communications 2007-08, Vol.359 (4), p.915-920
Main Authors: Shimizu, Satoshi, Kitada, Masaaki, Ishikawa, Hiroto, Itokazu, Yutaka, Wakao, Shohei, Dezawa, Mari
Format: Article
Language:English
Subjects:
Animals
Cell Culture Techniques - methods
Cell therapy
Cells, Cultured
Guided Tissue Regeneration - methods
Human mesenchymal cells
Humans
Mesenchymal Stem Cell Transplantation - methods
Nerve Regeneration - physiology
Peripheral nerve injury
Rats
Reconstruction
Schwann cells
Schwann Cells - cytology
Schwann Cells - transplantation
Sciatic Neuropathy - pathology
Sciatic Neuropathy - surgery
Tissue Engineering - methods
Transdifferentiation
Transplantation
Treatment Outcome
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Summary:We examined the availability of human bone marrow stromal cells (MSCs) as a source of transplantation therapy in nerve injury. Human MSCs were subjected to a series of treatments with a reducing agent, retinoic acid and a combination of trophic factors. Morphologically and immunocytochemically, such treated cells differentiated into Schwann cell characteristics in vitro. Cells were filled into a transpermeable tube, transplanted into the gap made in the rat sciatic nerve of a rat and followed up to 3 weeks under the control of immunosuppressant. In contrast to untreated human MSCs, differentiated human MSCs expressed Schwann cell markers in vivo and supported regenerating axons. These results suggest that human MSCs can be induced to be a substitute for Schwann cells that may be applied for nerve regeneration.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.05.212