Loading…
Differentiation of Streptomyces spp. which cause potato scab disease on the basis of partial rpoB gene sequences
In a previous study, we found that the phylogenetic analysis of partial rpoB sequences can be used effectively to phylogenetically differentiate Streptomyces spp. [B.J. Kim, C.J. Kim, J. Chun, Y.H. Koh, S.H. Lee, J.W. Hyun, C.Y. Cha, Y.H. Kook, Phylogenetic analysis of the genera Streptomyces and Ki...
Saved in:
Published in: | Systematic and applied microbiology 2007-07, Vol.30 (5), p.401-407 |
---|---|
Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | In a previous study, we found that the phylogenetic analysis of partial
rpoB sequences can be used effectively to phylogenetically differentiate
Streptomyces spp. [B.J. Kim, C.J. Kim, J. Chun, Y.H. Koh, S.H. Lee, J.W. Hyun, C.Y. Cha, Y.H. Kook, Phylogenetic analysis of the genera
Streptomyces and
Kitasatospora based on partial RNA polymerase beta-subunit gene (
rpoB) sequences, Int. J. Syst. Evol. Microbiol. 54 (2004) 593–598]. In the present study, we analyzed the partial
rpoB gene sequences of 19 reference
Streptomyces strains associated with potato scab. Furthermore, to empirically confirm the usefulness of
rpoB gene analysis for the phylogenetic differentiation of
Streptomyces spp., we applied the proposed system to 27 potato scab isolates obtained from the Korean provinces of Jeju-do and Kangwon-do. Phylogenetic relationships among these isolates using the devised
rpoB gene-based methods were generally similar to those reported for 16S rRNA gene-based analysis. Isolates from potato scab lesion in Korea were also clearly differentiated into their phylogenetic groups by this method. In addition, the deduced RpoB amino acid sequences were also found to be useful for differentiating these strains. Our data demonstrate that the
rpoB gene-based method can be used as a means of complementing other genetic methods such as 16S rRNA gene analysis or DNA–DNA hybridization to phylogenetically differentiate potato scab related
Streptomyces spp. |
---|---|
ISSN: | 0723-2020 1618-0984 |
DOI: | 10.1016/j.syapm.2007.01.003 |