Sequence polymorphism in the ribosomal DNA internal transcribed spacers differs among Theileria species

The genomic region spanning the two ribosomal RNA internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene was cloned and sequenced from sixteen Theileria isolates. Each Theileria species possessed ITS1 and ITS2 of unique size(s) and species specific nucleotide sequences. Varying degrees...

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Bibliographic Details
Published in:Veterinary parasitology 2007-07, Vol.147 (3-4), p.221-230
Main Authors: Aktas, Münir, Bendele, Kylie G., Altay, Kürsat, Dumanli, Nazir, Tsuji, Masayoshi, Holman, Patricia J.
Format: Article
Language:English
Subjects:
5.8S rRNA gene
Animals
Base Sequence
DNA, Ribosomal Spacer - genetics
genetic polymorphism
genetic variation
Internal transcribed spacer
internal transcribed spacers
Molecular Sequence Data
nucleotide sequences
Ovis
Phylogenetic tree
Phylogeny
Polymorphism
Polymorphism, Genetic
ribosomal DNA
Ribosomal RNA intergenic spacer
Ruminantia
sequence analysis
sequence homology
Theileria
Theileria - genetics
Theileria annulata
Theileria buffeli
Theileria mutans
Theileria parva
Theileria sergenti
Theileria species
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Summary:The genomic region spanning the two ribosomal RNA internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene was cloned and sequenced from sixteen Theileria isolates. Each Theileria species possessed ITS1 and ITS2 of unique size(s) and species specific nucleotide sequences. Varying degrees of ITS1 and ITS2 intra- and inter-species sequence polymorphism were found among ruminant Theileria species. The spacers were most polymorphic in the agent of tropical theileriosis, Theileria annulata, and were more conserved in two benign species, Theileria buffeli and Theileria sergenti Chitose. Phylogenetic analysis of the rDNA ITS1-5.8S rRNA gene-ITS2 region clearly separated each taxon, placing them in three clusters. One held T. annulata, Theileria parva, and Theileria mutans, with the latter two most closely related. The second held T. sergenti Ikeda, T. sergenti Chitose, and T. buffeli, with the latter two most closely related. The third cluster held the Theileria ovis isolates.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2007.04.007