Agmatine deiminase pathway genes in Lactobacillus brevis are linked to the tyrosine decarboxylation operon in a putative acid resistance locus

• Published in: Microbiology (Society for General Microbiology) 2007-07, Vol.153 (7), p.2221-2230
• Main Authors: Lucas, Patrick M, Blancato, Victor S, Claisse, Olivier, Magni, Christian, Lolkema, Juke S, Lonvaud-Funel, Aline
• Format: Article
• Language: English
• Subjects: Acids - pharmacology; Agmatine - metabolism; Arginine - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Decarboxylation; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Hydrolases - metabolism; Lactobacillus brevis; Lactobacillus brevis - drug effects; Lactobacillus brevis - genetics; Lactobacillus brevis - physiology; Microbiology; Molecular Sequence Data; Operon; Tyrosine - metabolism; Tyrosine Decarboxylase
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/mic.0.2007/006320-0

1 UMR 1219, Université Bordeaux 2, INRA, ISVV, Talence, France 2 Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands Correspondence Patrick M. Lucas patrick.lucas{at}oenologie.u-bordeaux2.fr In lactic acid bacteria (LAB), amino acids and their derivatives may be converted into amine-containing compounds designated biogenic amines, in pathways providing metabolic energy and/or acid resistance to the bacteria. In a previous study, a pathway converting tyrosine to tyramine was detected in Lactobacillus brevis and a fragment of a gene possibly involved in the production of another biogenic amine, putrescine, from agmatine, was detected in the same locus. The present study was carried out to determine if Lb. brevis actually harbours two biogenic amine-producing pathways in the same locus and to investigate the occurrence of the two gene clusters in other bacteria. Sequencing of the DNA locus in Lb. brevis revealed a cluster of six genes that are related to previously reported genes of agmatine deiminase pathways but with marked differences such as two genes encoding putative agmatine deiminases rather than one. Heterologous expression of encoded enzymes confirmed the presence of at least one active agmatine deiminase and one amino acid transporter that efficiently exchanged agmatine and putrescine. It was concluded that the Lb. brevis gene cluster encodes a functional and highly specific agmatine deiminase pathway. Screening of a collection of 197 LAB disclosed the same genes in 36 strains from six different species, and almost all the positive bacteria also contained the tyrosine catabolic pathway genes in the same locus. These results support the hypothesis that the agmatine deiminase and tyrosine catabolic pathways belong to a genomic region that provides acid resistance and that is exchanged horizontally as a whole between LAB. Abbreviations: AgDI, agmatine deiminase; AgmP, agmatine permease; LAB, lactic acid bacteria; TDC, tyrosine decarboxylation Present address: Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET) and Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531 (S2002LRK), Rosario, Argentina. The GenBank/EMBL/DDBJ accession number for the sequence determined in this work is AF446085. A supplementary table showing the distribution of AgDI and TDC gene clusters in LAB is available