Susceptibility of different cell lines to infection with bovine respiratory syncytial virus

The growth of bovine respiratory syncytial virus (BRSV) was evaluated in six different cell lines. Chicken embryo related cells (CER), a chicken embryo fibroblast/baby hamster kidney hybrid and bovine CRIB cells (a bovine viral diarrhea virus-resistant clone of MDBK cells) showed to be the most appr...

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Bibliographic Details
Published in:Journal of virological methods 2006-02, Vol.131 (2), p.130-133
Main Authors: Spilki, Fernando Rosado, de Almeida, Renata Servan, Campalans, Jacqueline, Arns, Clarice Weis
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bovine respiratory syncytial virus
BRSV
Cattle
Cell Line
Cell line susceptibility
Cercopithecus aethiops
Chick Embryo
Chickens
Cricetinae
Fundamental and applied biological sciences. Psychology
Microbiology
Respiratory Syncytial Virus, Bovine - growth & development
Techniques used in virology
Vero Cells
Virology
Virus Cultivation
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Summary:The growth of bovine respiratory syncytial virus (BRSV) was evaluated in six different cell lines. Chicken embryo related cells (CER), a chicken embryo fibroblast/baby hamster kidney hybrid and bovine CRIB cells (a bovine viral diarrhea virus-resistant clone of MDBK cells) showed to be the most appropriate for virus multiplication. Both cells provided infectious virus titres of up to 10 5.5 50% tissue culture infective doses per 100 μl (TCID 50/100 μl). One-step growth curves revealed no significant differences in the growth of BRSV in these two cell lines. Furthermore, they proved to be susceptible to infection with three different BRSV strains. It was concluded that both CER and CRIB cells may be used for laboratory multiplication of BRSV with optimal results.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2005.08.002