Differential expression of c-Met, its ligand HGF/SF and HER2/neu in DCIS and adjacent normal breast tissue

• Published in: Histopathology 2007-07, Vol.51 (1), p.54-62
• Main Authors: Lindemann, K, Resau, J, Nährig, J, Kort, E, Leeser, B, Annecke, K, Welk, A, Schäfer, J, Vande Woude, G F, Lengyel, E, Harbeck, N
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Breast - cytology; Breast - metabolism; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; c-Met; Carcinoma, Intraductal, Noninfiltrating - metabolism; Carcinoma, Intraductal, Noninfiltrating - pathology; DCIS; Female; Gene Expression Regulation, Neoplastic; Hepatocyte Growth Factor - genetics; Hepatocyte Growth Factor - metabolism; HER2/neu; HGF/SF; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Middle Aged; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Proto-Oncogene Proteins c-met - genetics; Proto-Oncogene Proteins c-met - metabolism; Receptor, ErbB-2 - genetics; Receptor, ErbB-2 - metabolism
• ISSN: 0309-0167; 1365-2559
• DOI: 10.1111/j.1365-2559.2007.02732.x

Aims:  Tyrosine kinase receptors Her2/neu and c‐Met play an important role in breast cancer development and progression. Our aim was to determine the expression of c‐Met, its ligand hepatocyte growth factor/scatter factor (HGF/SF) and Her2/neu in ductal carcinoma in situ (DCIS) lesions of the breast (n = 39) by two different immunocytochemical techniques, classical immunohistochemistry and immunofluorescence, and to correlate their expression levels with histopathological and clinical characteristics. Methods and results:  Both methods revealed similar c‐Met staining patterns in both the in situ component and the adjacent normal tissue (P