Long-term lowering of tumour interstitial fluid pressure reduces Ki-67 expression

Abstract High tumour interstitial fluid pressure (TIFP) is a characteristic of most solid tumours. Recent data give first evidence that mechanical stretch induced by TIFP triggers proliferation in solid tumours. In the present study we compared two protocols of TIFP reduction on the expression of th...

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Bibliographic Details
Published in:Journal of biomechanics 2007-01, Vol.40 (10), p.2324-2329
Main Authors: Hofmann, Matthias, Schultz, Maike, Bernd, August, Bereiter-Hahn, Jürgen, Kaufmann, Roland, Kippenberger, Stefan
Format: Article
Language:English
Subjects:
Animals
Catheterization
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation
Cysts
Extracellular Fluid - metabolism
Female
Gene Expression Regulation, Neoplastic
Ki-67
Ki-67 Antigen - biosynthesis
Kinases
Mechanical stretch
Mice
Mice, Nude
Neoplasms, Experimental - metabolism
Neoplasms, Experimental - pathology
Neoplasms, Experimental - therapy
Physical Medicine and Rehabilitation
Pressure
Quantitative analysis
Tumors
Tumour cell proliferation
Tumour interstitial fluid pressure
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Summary:Abstract High tumour interstitial fluid pressure (TIFP) is a characteristic of most solid tumours. Recent data give first evidence that mechanical stretch induced by TIFP triggers proliferation in solid tumours. In the present study we compared two protocols of TIFP reduction on the expression of the tumour proliferation marker Ki-67: (a) short-term lowering of TIFP by a singular puncture and (b) long-term lowering of TIFP by catheterization. Utilizing two experimental tumours (A431, A549) it was found that the TIFP broke down rapidly after a singular puncture but recovered within 6 h. In case of permanent catheterization no TIFP recovery was observed. After 24 h tumours were excised and stained against the proliferation marker Ki-67. While a singular puncture had no effect catheterized tumours showed a significant decrease in Ki-67 expression. Our data suggest that long-term lowering of TIFP is required to reduce tumour proliferation.
ISSN:0021-9290
1873-2380
DOI:10.1016/j.jbiomech.2006.10.039