Tubby protein in human lymphocytes from normal weight and obese subjects

The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared. 2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody. We fo...

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Bibliographic Details
Published in:Clinical biochemistry 2007-07, Vol.40 (11), p.806-809
Main Authors: Giannaccini, Gino, Giusti, Laura, Santini, Ferruccio, Marsili, Alessandro, Betti, Laura, Mascia, Giovanni, Pelosini, Caterina, Baroni, Stefano, Ciregia, Federica, Fabbrini, Laura, Lucacchini, Antonio, Vitti, Paolo, Pinchera, Aldo
Format: Article
Language:English
Subjects:
Adult
Body Weight
Echocardiography
Female
Health
Humans
Lymphocytes
Lymphocytes - metabolism
Male
Middle Aged
Obesity
Obesity - metabolism
Proteins - metabolism
Tubby protein
Two-dimensional electrophoresis
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Summary:The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared. 2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody. We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2007.03.020