Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection

Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic spe...

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Bibliographic Details
Published in:Molecular reproduction and development 2007-09, Vol.74 (9), p.1081-1088
Main Authors: Shinmen, Akie, Honda, Arata, Ohkawa, Mika, Hirose, Michiko, Ogonuki, Narumi, Yuzuriha, Misako, Miki, Hiromi, Mochida, Keiji, Inoue, Kimiko, Abe, Kuniya, Ito, Masao, Ogura, Atsuo
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
Cell Line
Chimera
Cryopreservation
DNA, Mitochondrial - genetics
Embryonic Development
Embryonic Stem Cells
Embryonic Structures
ES cells
Female
Genotype
Gynecology. Andrology. Obstetrics
ICSI
Male
Medical sciences
Mice
Mice, Mutant Strains - anatomy & histology
Mice, Mutant Strains - genetics
mitochondrial DNA
Mus musculus
Phenotype
placenta
polymorphism
Polymorphism, Genetic
Semen Preservation
Sperm Injections, Intracytoplasmic
Spermatozoa - cytology
Spermatozoa - physiology
Sterility. Assisted procreation
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Summary:Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI). Frozen‐thawed spermatozoa from wild‐derived strains, JF1 (Mus musculus molossinus), MSM (M. m. molossinus), HMI (M. m. castaneus), and SWN (M. m. spp.), were directly injected into mature oocytes from laboratory mice ([C57BL/6 × DBA2]F1; M. m. domesticus). The in vitro and in vivo developmental capacity of F1 embryos was not significantly different among the groups (P > 0.05), and term offspring were efficiently obtained in all groups (27%–34% of transferred embryos). However, the mean body and placental weights of the offspring differed significantly with genotype (P 
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.20612