Liposome Layers Characterized by Quartz Crystal Microbalance Measurements and Multirelease Delivery

Intact liposomes have been immobilized onto solid surfaces by a NeutrAvidin−biotin link. The construction of these layers has been followed up by X-ray photoelectron spectroscopy (XPS) and quartz crystal microbalance (QCM) measurements with energy dissipation monitoring. Also, the simultaneous relea...

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Bibliographic Details
Published in:Langmuir 2007-07, Vol.23 (14), p.7679-7686
Main Authors: Brochu, Heïdi, Vermette, Patrick
Format: Article
Language:English
Subjects:
Adsorption
Animals
Avidin - metabolism
Biosensing Techniques
Biotin - metabolism
Cattle
Chemistry
Colloidal state and disperse state
Culture Media - pharmacology
Drug Delivery Systems
Elasticity
Exact sciences and technology
Fetal Blood - chemistry
Fetal Blood - metabolism
Fluorescent Dyes - chemistry
General and physical chemistry
Kinetics
Liposomes - chemistry
Membranes
Quartz
Spectrum Analysis
Surface physical chemistry
Surface Properties
Viscosity
X-Rays
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Summary:Intact liposomes have been immobilized onto solid surfaces by a NeutrAvidin−biotin link. The construction of these layers has been followed up by X-ray photoelectron spectroscopy (XPS) and quartz crystal microbalance (QCM) measurements with energy dissipation monitoring. Also, the simultaneous release of two fluorescent probes from these liposome layers has been investigated with the aim to validate this method in multirelease delivery systems. XPS showed the successful immobilization of the different layers. XPS results also point out the importance of the deactivation method used to reveal the presence of the specific NeutrAvidin−biotin attachment. QCM measurements allowed the buildup of the different layers to be followed in real time and in situ and suggest that biotinylated liposomes stay intact upon surface attachment on NeutrAvidin-covered surfaces and had viscoelastic behavior. QCM experiments also demonstrated that surface-immobilized liposomes were able to resist irreversible adsorption from fetal bovine serum. Release kinetic profiles were studied by monitoring the release of two different fluorescent probes, namely, carboxyfluorescein and levofloxacin, from these liposome layers. These studies showed that it was possible to modulate to some extent the release rates of the two molecules by using different configurations of liposome layers.
ISSN:0743-7463
1520-5827
DOI:10.1021/la7003547