Isoelectric profiles of human erythropoietin are different in serum and urine

By adding a step of immunoaffinity to the method we had previously developed for analysing erythropoietin (EPO) in urine, we were able to study the isoelectric profiles of this hormone in human serum samples. This method was sensitive enough to investigate samples presenting physiological levels of...

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Bibliographic Details
Published in:International journal of biological macromolecules 2007-08, Vol.41 (3), p.354-357
Main Authors: Lasne, Françoise, Martin, Laurent, Martin, Jean Antoine, de Ceaurriz, Jacques
Format: Article
Language:English
Subjects:
Animals
Erythropoietin
Erythropoietin - blood
Erythropoietin - urine
Humans
Isoelectric Focusing
Isoelectric Point
Isoelectric profiles
Macaca fascicularis
Recombinant Proteins
Serum/urine comparison
Species Specificity
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Summary:By adding a step of immunoaffinity to the method we had previously developed for analysing erythropoietin (EPO) in urine, we were able to study the isoelectric profiles of this hormone in human serum samples. This method was sensitive enough to investigate samples presenting physiological levels of this hormone. Comparison with the corresponding profiles in urine showed that natural EPO was systematically more acidic in urine. The acidification process, which was not patent in the non-human primate Cynomolgus macaque, clearly also affected recombinant EPO when injected into humans. This process was unrelated to any enzymatic activity in urine since the incubation of natural or recombinant EPO in urine induced no transformation of their isoelectric profiles. The nature and mechanism of the structural modifications occurring during the renal handling of this hormone remain to be investigated.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2007.04.002