The impact of a cation channel blocker (furosemide) on Pseudomonas aeruginosa PAO1 biofilm architecture

To determine if furosemide could have a role in inhibition of biofilm formation. A prospective study of 72-hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls. Bacteria were treated with furosemide and controls to determine its antiplanktonic properties. PA01...

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Bibliographic Details
Published in:Otolaryngology-head and neck surgery 2007-07, Vol.137 (1), p.21-26
Main Authors: Cross, Jenny L., Ramadan, Hassan H., Thomas, John G.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bacteriological Techniques - instrumentation
Biofilms - drug effects
Biofilms - growth & development
Drug Resistance, Bacterial
Furosemide - pharmacology
Humans
Hydrogen-Ion Concentration
Microbial Sensitivity Tests
Prospective Studies
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - physiology
Sodium Potassium Chloride Symporter Inhibitors - pharmacology
Time Factors
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Summary:To determine if furosemide could have a role in inhibition of biofilm formation. A prospective study of 72-hour biofilms grown in the Calgary Biofilm Device (CBD) treated with furosemide and controls. Bacteria were treated with furosemide and controls to determine its antiplanktonic properties. PA01 biofilms were treated with serial 2-fold dilutions of furosemide and controls over a 48-hour period at pH concentrations of 7.0, 8.0, and 9.0. Furosemide had no effect on planktonic PA01. It had minimal effect at pH below 8.0. A pH of 8.0 and 9.0 reduced biofilms 25%. Furosemide 10 mg/mL reduced biofilms 50% at pH 8.0 and 9.0. It inhibited biofilms to
ISSN:0194-5998
1097-6817
DOI:10.1016/j.otohns.2007.02.022