Method for determining the average degree of substitution of o-vanillin derivatized porcine somatotropin

Electrospray mass spectral observation directly on a sample of a derivatized protein, such as porcine somatotropin (pST), affords a method for evaluating the degree of substitution of this protein. Derivatization of the lysine residues and the terminal amino residue here by formation of a Schiff bas...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2006-01, Vol.40 (1), p.185-189
Main Authors: Sharp, Thomas R., Morris, Ronald, Horan, George J., Pezzullo, Linda H., Stroh, Justin G.
Format: Article
Language:English
Subjects:
Aldehydes - analysis
Amino Acid Sequence
Analysis
Analytical, structural and metabolic biochemistry
Animals
Average degree of substitution measurement
Benzaldehydes - analysis
Biological and medical sciences
Chemistry Techniques, Analytical - methods
Chemistry, Pharmaceutical - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Growth Hormone - analysis
Growth Hormone - chemistry
Lysine - analysis
Lysine - chemistry
Mass Spectrometry
Medical sciences
Models, Chemical
Molecular Sequence Data
o-Vanillin
Pharmacology. Drug treatments
Porcine somatotropin
Protein derivatization
Protein Structure, Tertiary
Spectrometry, Mass, Electrospray Ionization - methods
Swine
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Summary:Electrospray mass spectral observation directly on a sample of a derivatized protein, such as porcine somatotropin (pST), affords a method for evaluating the degree of substitution of this protein. Derivatization of the lysine residues and the terminal amino residue here by formation of a Schiff base with a small aromatic aldehyde (in this case, o-vanillin) affords stabilization of the protein so that it may be used in a controlled release veterinary pharmaceutical formulation. This method permits direct observation of substitutions, optimization of manufacturing procedures for producing a commercial product, and permits quality evaluation of material.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2005.06.028