High resolution imaging of fluorescein patterns in RCS rat retinae and their direct correlation with histology

To assess the progressive changes in the retinal vascular bed of dystrophic and non-dystrophic Royal College of Surgeons (RCS) rats, retinae, were visualised correlating in vivo fundus fluorescein angiography (FA) with histology. FA was performed in rats aged 5 weeks to 2 years, using a Zeiss confoc...

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Bibliographic Details
Published in:Experimental eye research 2006, Vol.82 (1), p.164-171
Main Authors: Zambarakji, H.J., Keegan, D.J., Holmes, T.M., Halfyard, A.S., Villegas-Perez, M.P., Charteris, D.G., Fitzke, F.W., Greenwood, J., Lund, R.D.
Format: Article
Language:English
Subjects:
Animals
confocal scanning ophthalmoscope
Disease Progression
Fluorescein Angiography
Microscopy, Confocal
Muscular Dystrophies - pathology
Rats
Rats, Mutant Strains
RCS rats
retina
Retina - pathology
Retinal Degeneration - pathology
Retinal Ganglion Cells - pathology
Retinal Vessels - pathology
vascular
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Summary:To assess the progressive changes in the retinal vascular bed of dystrophic and non-dystrophic Royal College of Surgeons (RCS) rats, retinae, were visualised correlating in vivo fundus fluorescein angiography (FA) with histology. FA was performed in rats aged 5 weeks to 2 years, using a Zeiss confocal scanning laser ophthalmoscope (cSLO). After the final imaging session, a subset of retinae were prepared for flat-mount histology and the vascular bed was visualised using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) staining. While non-dystrophic rat retinae showed no substantive changes in vascular patterns with age and no demonstrable fluorescein leakage up to at least 1 year, dystrophic rat retinae showed abnormal vascular formations, demonstrable on FA and NADPH-d staining, which could be correlated in single retinae. Hyperfluorescent spots and late angiographic leakage were evident beginning at 10 weeks and progressed in severity with time: they were coincident in distribution with abnormal histological vascular complexes. The ability to monitor the same retina serially makes this approach a valuable tool for studying the dynamics of vascular change in the diseased retina, not only during the course of degeneration but also when assessing efficacy of potential therapeutic approaches.
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2005.06.006