Structure and assembly of epiglucan, the extracellular (1→3;1→6)-β-glucan produced by the fungus Epicoccum nigrum strain F19

In a previous article [ Carbohydr. Res. 2001, 331, 163–171] two different structures for the possible modular repeating unit of the extracellular β-glucan, epiglucan produced by the fungus Epicoccum nigrum strain F19 were proposed. Clarifying which was the more likely one was considered essential be...

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Bibliographic Details
Published in:Carbohydrate research 2006-02, Vol.341 (3), p.365-373
Main Authors: Schmid, Frank, Stone, Bruce A., Brownlee, Robert T.C., McDougall, Barbara M., Seviour, Robert J.
Format: Article
Language:English
Subjects:
Ascomycota - chemistry
beta-Glucans - chemistry
beta-Glucans - metabolism
Biosynthesis
Carbohydrate Conformation
Carbohydrate Sequence
Carbon Radioisotopes
Chromatography, Thin Layer
Epiglucan elongation
Extracellular polysaccharide
Glucan 1,3-beta-Glucosidase - metabolism
Glucans - chemistry
Glucans - isolation & purification
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
NMR spectroscopy
Radioisotope labelling
Side chain insertion
Spectrophotometry, Infrared
β-Glucan assembly
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Summary:In a previous article [ Carbohydr. Res. 2001, 331, 163–171] two different structures for the possible modular repeating unit of the extracellular β-glucan, epiglucan produced by the fungus Epicoccum nigrum strain F19 were proposed. Clarifying which was the more likely one was considered essential before attempts were made to understand how epiglucan was assembled by this fungus. Data from Smith degradation analyses of epiglucan were consistent with the repeating unit of structure I, where single glucosyl residues are attached by (1→6)-β-linkages to two out of every three glucosyl residues in the (1→3)-β-linked glucan backbone. Repeated Smith degradations of 14C-glucose labelled epiglucan showed that chain elongation occurred from its non-reducing end. Side chain insertion into the growing glucan was followed by analysis of real time incorporation of 13C-glucose into epiglucan by 13C NMR, and 14C-glucose by enzymic digestion of the synthesised 14C-epiglucan. All data obtained were consistent with the view that single (1→6)-β-linked glucosyl side residues are inserted simultaneously as the glucan backbone elongates. ▪
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2005.10.013