Pentoxifylline inhibits mature burn scar fibroblasts in culture

Fibroblasts are thought to be (in part) responsible for the persisting contractile forces that result in burn contractures. Using monolayer and fibroblast populated collagen lattice (FPCL) models we subjected burn scar fibroblasts to the anti-fibrinolytic agent Pentoxifylline (PFX) in an attempt to...

Full description

Saved in:
Bibliographic Details
Published in:Burns 2006-02, Vol.32 (1), p.42-45
Main Authors: Rawlins, J.M., Lam, W.L., Karoo, R.O., Naylor, I.L., Sharpe, D.T.
Format: Article
Language:English
Subjects:
Burn wound
Burns - complications
Cells, Cultured
Cicatrix, Hypertrophic - prevention & control
Contracture - etiology
Contracture - prevention & control
Dose-Response Relationship, Drug
Fibroblast
Fibroblasts - drug effects
Hematologic Agents - administration & dosage
Humans
Pentoxifylline - administration & dosage
Pentoxifyllines
Scar
Treatment Outcome
Wound healing
Wound Healing - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fibroblasts are thought to be (in part) responsible for the persisting contractile forces that result in burn contractures. Using monolayer and fibroblast populated collagen lattice (FPCL) models we subjected burn scar fibroblasts to the anti-fibrinolytic agent Pentoxifylline (PFX) in an attempt to reduce proliferation and contraction of these cells. Fibroblasts were isolated from mature burn scars at reconstructive surgery. Fibroblasts were grown in monolayer or incorporated into FPCL's and exposed to PFX. Fibroblast numbers and FPCL surface areas were calculated using digital photography and image analysis. PFX showed a dose-dependent inhibition of contraction and reduced proliferation of burn scar fibroblasts. In monolayer, cell number proliferation was markedly reduced. FPCL's containing 0, 0.25, 0.5, 1, and 2 mg/ml of PFX had relative surface areas of 31, 40, 43, 59, and 85%, respectively. One and 2 mg/ml FPCL's contracted significantly less than controls ( p < 0.0001). This is the first study to show the dose-dependent effects of Pentoxifylline on the proliferation and contraction of burn scar fibroblasts. This study suggests that Pentoxifylline has a direct effect on inhibiting burn scar fibroblasts. Further study of PFX on burn scars will provide opportunities to reduce burn scar contractures in vivo.
ISSN:0305-4179
1879-1409
DOI:10.1016/j.burns.2005.08.004