Requirement of MMP-3 in anchorage-independent growth of oral squamous cell carcinomas

Background:  Matrix metalloproteinase‐3 (MMP‐3) is expressed in various carcinomas; however, its function is not clearly established. This study was to assess its possible role in oral squamous cell carcinomas (OSCCs). Materials and methods:  Specimens of seven oral pre‐malignant lesions (OPMLs) and...

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Published in:Journal of oral pathology & medicine 2007-08, Vol.36 (7), p.430-435
Main Authors: Liu, Shyun-Yeu, Liu, Young-Chau, Huang, Wen-Tsung, Huang, Guan-Cheng, Su, Hsiao-Jing, Lin, Mei-Huei
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carcinoma - genetics
Carcinoma - pathology
Carcinoma, Squamous Cell - enzymology
Cell Count
Cell Division - genetics
Cell Line, Tumor
Dentistry
DNA, Antisense - genetics
esophageal carcinoma
Esophageal Neoplasms - genetics
Esophageal Neoplasms - pathology
Esophagus
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression Regulation, Neoplastic - genetics
Humans
Indicators and Reagents
Matrix Metalloproteinase 3 - genetics
Matrix Metalloproteinase 3 - physiology
matrix metalloproteinase-3
Medical sciences
Mouth Neoplasms - enzymology
oral squamous cell carcinoma
Otorhinolaryngology. Stomatology
Precancerous Conditions - enzymology
stromelysin-1
Tetrazolium Salts
Transfection
Tumors
Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
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Summary:Background:  Matrix metalloproteinase‐3 (MMP‐3) is expressed in various carcinomas; however, its function is not clearly established. This study was to assess its possible role in oral squamous cell carcinomas (OSCCs). Materials and methods:  Specimens of seven oral pre‐malignant lesions (OPMLs) and 92 OSCCs were subjected to MMP‐3 detection by RT‐PCR and Western blot. Antisense oligodeoxynucleotides (AODNs) of MMP‐3 were used to transfect OSCC (OECM‐1 and SCC‐9) and esophageal carcinoma (CE81T/VGH) cell lines, and their growth was subsequently analyzed by XTT and soft‐agar colony assay. Results:  MMP‐3 transcript was preferentially expressed in OSCCs (71 of 92, 77%) than in OPMLs (two of seven, 29%; P = 0.012). Both MMP‐3 transcript and protein levels were significantly higher in OSCC masses than in neighboring tissues (P 
ISSN:0904-2512
1600-0714
DOI:10.1111/j.1600-0714.2007.00524.x